CD90/Thy1 Antibody (OX-7) [DyLight 550]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65543R

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse, Rat

Applications

ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

DyLight 550 (Excitation = 562 nm, Emission = 576 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # OX-7

Concentration

1.0 mg/ml

View all CD90/Thy1 Antibodies »

Product Specifications

Immunogen

Rat Thy 1 antigen

Reactivity Notes

Reacts with: Rabbit, Mouse, Guinea Pig. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

CD90

Marker

Mesenchymal Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for CD90/Thy1 Antibody (OX-7) [DyLight 550]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD90/Thy1

CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1. Thy1.1, expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues. Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab' of MRC OX-7 for rat Thy1 is 3 x 10^9m^1 and for mouse Thy1.1 is 3 x 10^8m-^1(1). Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy (5).This clone has been reported to induce glomerular nephritis in Wistar rats (8).This product is routinely tested in flow cytometry on rat thymocytes.

Alternate Names

CD90, Thy1

Gene Symbol

THY1

Additional CD90/Thy1 Products

Product Documents for CD90/Thy1 Antibody (OX-7) [DyLight 550]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD90/Thy1 Antibody (OX-7) [DyLight 550]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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