CD90/Thy1 Antibody (OX-7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80661

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OX-7

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD90/Thy1 Antibodies »

Product Specifications

Immunogen

Rat Thy 1 antigen

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD90/Thy1 Antibody (OX-7) - Azide and BSA Free

Flow Cytometry: CD90/Thy1 Antibody (OX-7) - Azide and BSA Free [NBP2-80661]

Flow Cytometry: CD90/Thy1 Antibody (OX-7) - Azide and BSA Free [NBP2-80661]

Flow Cytometry: CD90/Thy1 Antibody (OX-7) - Azide and BSA Free [NBP2-80661] - Staining of rat thymus with MOUSE ANTI RAT CD90 (THY 1.1). Image from the standard format of this antibody.
Flow Cytometry: CD90/Thy1 Antibody (OX-7) - Azide and BSA Free [NBP2-80661]

Flow Cytometry: CD90/Thy1 Antibody (OX-7) - Azide and BSA Free [NBP2-80661]

Flow Cytometry: CD90/Thy1 Antibody (OX-7) - Azide and BSA Free [NBP2-80661] - Staining of rat thymocytes. Image from the standard format of this antibody.
Flow Cytometry: CD90/Thy1 Antibody (OX-7) - Azide and BSA Free [NBP2-80661]

Flow Cytometry: CD90/Thy1 Antibody (OX-7) - Azide and BSA Free [NBP2-80661]

Flow Cytometry: CD90/Thy1 Antibody (OX-7) - Azide and BSA Free [NBP2-80661] - Staining of rat thymocytes. Image from the standard format of this antibody.
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Applications for CD90/Thy1 Antibody (OX-7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

: 1:50-1:100

Immunocytochemistry/Immunofluorescence

1:10-1:500

Immunohistochemistry-Frozen

: 1:10-1:500

Immunoprecipitation

: 1:10-1:500

Western Blot

: 1:100-1:2000
Application Notes
For Flow Cytometry: Use 10 uL of the suggested working dilution to label 10^6 cells in 100 uL.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD90/Thy1

CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1. Thy1.1, expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues. Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab' of MRC OX-7 for rat Thy1 is 3 x 10^9m^1 and for mouse Thy1.1 is 3 x 10^8m-^1(1). Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy (5).This clone has been reported to induce glomerular nephritis in Wistar rats (8).This product is routinely tested in flow cytometry on rat thymocytes.

Alternate Names

CD90, Thy1

Gene Symbol

THY1

Additional CD90/Thy1 Products

Product Documents for CD90/Thy1 Antibody (OX-7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD90/Thy1 Antibody (OX-7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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