Human/Mouse/Rat beta-Catenin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1329
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
ICC, IHC-P, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 196621
Product Summary for Human/Mouse/Rat beta-Catenin Antibody
Immunogen
E. coli-derived recombinant human beta-Catenin
Ala2-Leu781
Accession # P35222
Ala2-Leu781
Accession # P35222
Specificity
Detects rh beta-Catenin and endogenous human, mouse and rat beta-Catenin in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat beta-Catenin Antibody
Detection of Human/Mouse/Rat beta-Catenin by Western Blot.
Western blot shows lysates of Huh-7 human hepatoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat beta-Catenin Monoclonal Antibody (Catalog # MAB1329) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for beta-Catenin at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
beta‑Catenin in Human Pancreas.
beta-Catenin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human/Mouse/Rat beta-Catenin Monoclonal Antibody (Catalog # MAB1329) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse/Rat beta-Catenin Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Western Blot
2 µg/mL
Sample: Huh-7 human hepatoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line
Sample: Huh-7 human hepatoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 3.5 using MAB1329 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-Catenin
Alternate Names
bCatenin, CTNNB1
Gene Symbol
CTNNB1
UniProt
Additional beta-Catenin Products
Product Documents for Human/Mouse/Rat beta-Catenin Antibody
Product Specific Notices for Human/Mouse/Rat beta-Catenin Antibody
For research use only
Loading...
Loading...
Loading...
Loading...