Human/Mouse/Rat Caveolin-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5736
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Flow Cytometry, ICC, IHC, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 7C8
Product Summary for Human/Mouse/Rat Caveolin-1 Antibody
Immunogen
Puified rat adipocyte low density microsomes
Accession # P41350
Accession # P41350
Specificity
Detects endogenous human, mouse and rat Caveolin-1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat Caveolin-1 Antibody
Detection of Human Caveolin-1 by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells and HMVEC human microvascular endothelial cells. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Caveolin-1 Monoclonal Antibody (Catalog # MAB5736) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caveolin-1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Caveolin‑1 in A431 Human Cell Line.
Caveolin-1 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Caveolin-1 Monoclonal Antibody (Catalog # MAB5736) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat Caveolin-1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells and HMVEC human microvascular endothelial cells
Sample: HUVEC human umbilical vein endothelial cells and HMVEC human microvascular endothelial cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB5736 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caveolin-1
Alternate Names
CAV1, Caveolin1, MSTP085, VIP21
Gene Symbol
CAV1
UniProt
Additional Caveolin-1 Products
Product Documents for Human/Mouse/Rat Caveolin-1 Antibody
Product Specific Notices for Human/Mouse/Rat Caveolin-1 Antibody
For research use only
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