Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2B Clone # 7C8

View all Caveolin-1 Antibodies »

Product Summary for Human/Mouse/Rat Caveolin-1 Biotinylated Antibody

Immunogen

Purified rat adipocyte low density microsomes
Accession # P41350

Specificity

Caveolin-1 antibodies are ideal for immunocytochemistry colocalization studies in caveolae. The unconjugated antibody detects endogenous human, mouse and rat Caveolin-1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat Caveolin-1 Biotinylated Antibody

Caveolin-1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Caveolin-1 in HeLa Human Cell Line.

Caveolin-1 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Caveolin-1 Biotinylated Monoclonal Antibody (Catalog # BAM5736) at 25 µg/mL overnight at 4 °C. Cells were stained using the NorthernLights™ 493-conjugated Streptavidin (green; Catalog # NL997) and counterstained with DAPI (blue). Specific staining was localized to caveolae. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat Caveolin-1 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Formaldehyde fixed HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and Sodium Azide with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caveolin-1

Caveolin-1 is a palmitoylated 22 kDa membrane-associated protein in caveolae, the cholesterol-rich invaginations in the plasma membrane involved in vesicular transport and regulation of lipid rafts. Caveolin-1 expression is dysregulated during cancer progression and exhibits both positive and negative effects on tumor progression. The central region of Caveolin-1 (amino acids 105‑125) is buried in the lipid layer, while the N- and C-terminal flanking regions are exposed to the cytoplasm and interact with many other proteins. Within these cytoplasmic regions, human Caveolin-1 shares 95% amino acid sequence identity with mouse and rat Caveolin-1. Alternate splicing in human, mouse and rat generates an isoform with a deletion of the N-terminal 31 residues.

Alternate Names

CAV1, Caveolin1, MSTP085, VIP21

Entrez Gene IDs

857 (Human); 12389 (Mouse); 25404 (Rat)

Gene Symbol

CAV1

UniProt

P41350

Additional Caveolin-1 Products

Product Documents for Human/Mouse/Rat Caveolin-1 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat Caveolin-1 Biotinylated Antibody

For research use only

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