Human/Mouse/Rat CD31/PECAM-1 Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # AF3628
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Antibody Source
Product Summary for Human/Mouse/Rat CD31/PECAM-1 Antibody
Immunogen
Glu18-Lys590
Accession # Q08481
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse/Rat CD31/PECAM-1 Antibody
Detection of Mouse CD31/PECAM‑1 by Western Blot.
Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Detection of CD31/PECAM‑1 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or control antibody (AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108).Detection of CD31/PECAM‑1 in Rat Splenocytes by Flow Cytometry.
Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108).Applications for Human/Mouse/Rat CD31/PECAM-1 Antibody
CyTOF-ready
Flow Cytometry
Sample: Mouse and rat splenocytes, HUVEC cells
Immunocytochemistry
Sample: Immersion fixed bEnd.3 mouse endothelioma cell line
Immunohistochemistry
Sample: Immersion fixed frozen sections of mouse embryo (13.5 d.p.c. and 14 d.p.c.), rat heart and immersion fixed paraffin-embedded sections of Human Placenta
Western Blot
Sample: bEnd.3 mouse endothelioma cell line
Reviewed Applications
Read 20 reviews rated 5 using AF3628 in the following applications:
- CLARITY deep tissue imaging (1 Review)
- Free floating vibratome sections of 80microns and staining for brain clearing purposes where methanol treatment was used, the antibody still worked really good. (1 Review)
- Immunocytochemistry (2 Reviews)
- Immunocytochemistry/Immunofluorescence (6 Reviews)
- Immunohistochemistry (6 Reviews)
- Immunohistochemistry-Frozen (2 Reviews)
- Simple Western (1 Review)
- strong staining at 1/100 secondary AF647 (1 Review)
Formulation, Preparation, and Storage
Purification
Endotoxin Level
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD31/PECAM-1
PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM-/- mice are deficient in chemokine-mediated chemotaxis (14).
References
- Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
- Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
- Liao, F. et al. (1997) J. Exp. Med. 185:1349.
- Nakada, M.T. et al. (2000) J. Immunol. 164:452.
- Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
- Ilan, N. et al. (2001) FASEB J. 15:362.
- Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
- Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
- Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
- Mamdouh, Z. et al. (2003) Nature 421:748.
- Gao, C. et al. (2003) Blood 102:169.
- Falati, S. et al. (2006) Blood 107:535.
- Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
- Wu, Y. et al. (2005) J. Immunol. 175:3484.
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Additional CD31/PECAM-1 Products
Product Documents for Human/Mouse/Rat CD31/PECAM-1 Antibody
Product Specific Notices for Human/Mouse/Rat CD31/PECAM-1 Antibody
For research use only