Detects human TREML1/TLT‑1 in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant mouse TREML1 or recombinant human TREML2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Human TREML1/TLT-1 Antibody

Detection of TREML1/TLT‑1 in Human Platelets by Flow Cytometry.

Human peripheral blood platelets were stained with Mouse Anti-Human Integrin a2b/CD41 PE-conjugated Monoclonal Antibody (Catalog # FAB7616P) and either (A) Rat Anti-Human TREML1/TLT-1 Monoclonal Antibody (Catalog # MAB2394) or (B) Rat IgG_2BIsotype Control (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TREML1/TLT-1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood platelets

Western Blot

1 µg/mL
Sample: Recombinant Human TREML1/TLT-1

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREML1/TLT-1

TREML1 is an inhibitory type I transmembrane protein that has two cytoplasmic ITIMs (Immunoreceptor Tyrosine-based Inhibition Motif). A splice variant lacking ITIMs in its cytoplasmic domain also exists. TREML1 is found only in platelets and megakaryocytes.

Long Name

Triggering Receptor Expressed on Myeloid Cells-like 1

Alternate Names

TLT-1, TLT1

Entrez Gene IDs

340205 (Human); 71326 (Mouse)

Gene Symbol

TREML1

UniProt

Q86YW5

Additional TREML1/TLT-1 Products

Product Documents for Human TREML1/TLT-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TREML1/TLT-1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TREML1/TLT-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2394

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all TREML1/TLT-1 Antibodies »

Product Summary for Human TREML1/TLT-1 Antibody

Immunogen

Specificity