SARS Nucleocapsid Protein Antibody Pack

Name: SARS Nucleocapsid Protein Antibody Pack
Brand: Novus Biologicals
SKU: NBP3-05695

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05695

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Product Summary
Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

SARS-CoV, SARS-CoV-2

Applications

Dual RNAscope ISH-IHC, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Format

Sample-size Vials

Product Summary for SARS Nucleocapsid Protein Antibody Pack

This pack contains 1 vial each of: NBP2-44205-25ug (25 ug), NB100-56576SS (25 ug), NB100-56683SS (25 ug). NBP2-44205 is manufactured exclusively by Rockland Immunochemicals.

Product Specifications

Clonality

Polyclonal

Application Notes

See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Scientific Data Images for SARS Nucleocapsid Protein Antibody Pack

Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56576] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.

Immunocytochemistry/Immunofluorescence: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody[NBP2-44205], showing viral protein synthesis. VeroE6 cells were infected with rSARS-CoV-2. Mock: non-infected cells. At 48 h.p.i, cells were fixed and prepared for immunofluorescence staining with primary antibodies directed against double-stranded RNA (dsRNA) [green] and SARS-CoV Nucleocapsid (N) [red]. Nuclear Counterstain DAPI [blue].

Western Blot: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody [NB100-56683] at a 1:2000 dilution.

Western Blot: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Western blot using Protein A Purified anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46 kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody[NBP2-44205] at room temperature for 1 h followed by detection using IRDy(TM) 800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:10,000. The fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56683] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.

Immunohistochemistry: SARS Nucleocapsid Protein Antibody Pack [NBP3-05695] - Pathological changes in rhesus macaques infected with SARS-CoV-2. (g) SARSCoV-2 antigen is detected by immunohistochemistry in type I pneumocytes. Magnification 400x. (j) SARS-CoV-2 antigen is detected by immunohistochemistry in type I pneumocytes (asterisk) and type II pneumocytes (arrow) as well as alveolar macrophages (arrowheads). Magnification 400x. (k) SARS-CoV-2 antigen is detected by immunohistochemistry in mediastinal lymph node. Magnification 400x. (l) SARSCoV-2 antigen is detected by immunohistochemistry in macrophages and lymphocytes in the lamina propria of the cecum. Magnification 400x. bioRxiv March 21, 2020 //doi.org/10.1101/2020.03.21.001628 SARS Nucleocapsid Protein Antibody [NB100-56576]

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Kit Contents for SARS Nucleocapsid Protein Antibody Pack

NBP2-44205 (SARS-Nucleocapsid protein Antibody) - 100 ug
HAF008: Rabbit IgG Horseradish Peroxidase-conjugated Antibody
NB100-56683: SARS Nucleocapsid Protein Antibody
NB100-56576: SARS Nucleocapsid Protein Antibody - BSA Free

Formulation, Preparation, and Storage

Formulation

NB100-56576 and NB100-56683: PBS, 0.05% Sodium Azide. NBP2-44205: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% Sodium Azide.

Concentration

Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS Nucleocapsid Protein

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139 kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Alternate Names

N, N protein, N structural protein, NC, Nucleocapsid protein, Nucleoprotein, Protein N, SARS coronavirus N protein, SARS coronavirus nucleocapsid protein, SARS CoV, SARS CoV N protein, SARS CoV nucleocapsid protein, SARS N protein, SARS Nucleoprotein, SARSCoV, SARSCoV N protein, SARSCoV nucleocapsid protein, Severe acute respiratory syndrome, 2019-nCoV Nucleocapsid Protein, COVID-19 N protein, COVID-19 nucleocapsid protein, SARSCOV2 N protein, SARS-CoV-2 N protein, SARS-COV-2 nucleocapsid protein, SARS-COV-2 Nucleoprotein

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Additional SARS Nucleocapsid Protein Products

Product Documents for SARS Nucleocapsid Protein Antibody Pack

Product Datasheets

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COA

Product Specific Notices for SARS Nucleocapsid Protein Antibody Pack

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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