TrkB Antibody (OTI2E1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47898
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
ICC/IF, IHC-P
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI2E1
Concentration
1.17 mg/ml
Product Summary for TrkB Antibody (OTI2E1)
Immunogen
Full-length protein expressed in 293T cell transfected with human NTRK2 expression vector
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
This antibody is specific for Homo sapiens neurotrophic tyrosine kinase, receptor, type 2 (NTRK2), transcript variant b.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TrkB Antibody (OTI2E1)
Western Blot: TrkB Antibody (2E1) [NBP1-47898] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY NTRK2 (RC205129, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiNTRK2.
Immunocytochemistry/Immunofluorescence: TrkB Antibody (2E1) [NBP1-47898] Staining of COS7 cells transiently transfected by pCMV6-ENTRY TrkB.
Immunohistochemistry-Paraffin: TrkB Antibody (2E1) [NBP1-47898] - Staining of paraffin-embedded Human lung tissue using anti-TrkB mouse monoclonal antibody.
Applications for TrkB Antibody (OTI2E1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.17 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TrkB
Long Name
Neurotrophic Tyrosine Kinase Receptor B
Alternate Names
NTRK2
Entrez Gene IDs
4915 (Human)
Gene Symbol
NTRK2
Additional TrkB Products
Product Documents for TrkB Antibody (OTI2E1)
Product Specific Notices for TrkB Antibody (OTI2E1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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