Cycloheximide

Name: Cycloheximide
Brand: Tocris Bioscience
SKU: 0970
Rating: 5 (3 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 0970

(3)

Citations (129)

COA / SDS

Inhibitor of protein synthesis

Key Product Details

Description:	Inhibitor of protein synthesis
Chemical Name:	4-[2-(3,5-Dimethyl-2-oxo-cyclohexyl)-2-hydroxyethyl]-2,6-piperidinedione
Purity:	≥97% (HPLC)
Molecular Weight:	281.35

View Full Technical Details for Cycloheximide

Technical Data
Citations / Reviews (3)

Biological Activity for Cycloheximide

Cycloheximide is a selective inhibitor of eukaryotic (over prokaryotic) protein synthesis, blocking tRNA binding and release from ribosomes. Induces apoptosis in a variety of transformed and normal cell lines, including T cells. Competitively inhibits the PPIase hFKBP12 (K_i = 3.4 μM). Also inhibits ferroptosis. Antifungal antibiotic. Exhibits anti-MERS-CoV activity in Vero cells in vitro (IC₅₀ = 0.16 μM).

Technical Data for Cycloheximide

M. Wt	281.35
Formula	C₁₅H₂₃NO₄
Storage	Desiccate at RT
Purity	≥97% (HPLC)
CAS Number	66-81-9
PubChem ID	6197
InChi Key	YPHMISFOHDHNIV-FSZOTQKASA-N
Smiles	O=C(N1)CC(C[C@@H](O)[C@]2([H])C[C@@H](C)C[C@H](C)C2=O)CC1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Cycloheximide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	14.07	50
	water	7.03	25

Preparing Stock Solutions for Cycloheximide

The following data is based on the product molecular weight 281.35.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	7.11 mL	35.54 mL	71.09 mL
2.5 mM	1.42 mL	7.11 mL	14.22 mL
5 mM	0.71 mL	3.55 mL	7.11 mL
25 mM	0.14 mL	0.71 mL	1.42 mL

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Reconstitution Calculator

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References for Cycloheximide

References are publications that support the biological activity of the product.

Sawicki and Sawicki Coronavirus minus-strand RNA synthesis and effect of cycloheximide on coronavirus RNA synthesis. J.Virol. 1986 PMID: 2867230
Ko Screening of FDA-approved drugs using a MERS-CoV clinical isolate from South Korea identifies potential therapeutic options for COVID-19. Viruses 2021 PMID: 33918958
Xie Ferroptosis: process and function. Cell.Death.Differ. 2016 PMID: 26794443
Tang Cycloheximide-induced T-cell death is mediated by a Fas-associated death domain-dependent mechanism. J.Biol.Chem. 1999 PMID: 10066786
Obrig The mechanism by which cycloheximide and related glutarimide antibiotics inhibit peptide synthesis on reticulocyte ribosomes. J.Biol.Chem. 1971 PMID: 5541758
Christner Synthesis and cytotoxic evaluation of cycloheximide derivatives as potential inhibitors of FKBP12 with neuroregenerative properties. J.Med.Chem. 1999 PMID: 10479292

Product Documents for Cycloheximide

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.

SDS

⚠ WARNING: This product can expose you to chemicals including Cycloheximide, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov