Lin28 1632

Name: Lin28 1632
Brand: Tocris Bioscience
SKU: 6068
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 6068

(1)

Citations (3)

COA / SDS

RNA binding protein Lin28 inhibitor; promotes mESC differentiation; also bromodomain inhibitor

Key Product Details

Description:	RNA binding protein Lin28 inhibitor; promotes mESC differentiation; also bromodomain inhibitor
Chemical Name:	N-Methyl-N-[3-(3-methyl-1,2,4-triazolo[4,3-b]pyridazin-6-yl)phenyl]acetamide
Purity:	≥98% (HPLC)
Molecular Weight:	281.31

View Full Technical Details for Lin28 1632

Technical Data
Citations / Reviews (1)

Biological Activity for Lin28 1632

Lin28 1632 is an RNA binding protein Lin28 inhibitor (IC₅₀ = 8 μM). Lin28 1632 blocks binding of Lin28 to let-7 pre-micro RNA. Lin28 1632 inhibits stemness and induces differentiation of murine ESCs. Lin28 1632 increases let-7 miRNA and suppresses PD-L1 expression in multiple human cancer cell lines. Lin28 1632 inhibits growth of tumor cell lines and tumor-sphere formation in vitro. Lin28 1632 also inhibits growth of tumor xenografts in mice by enhancing T cell-mediated antitumor immunity. Lin28 1632 is also bromodomain inhibitor. Lin28 1632 inhibits tumor growth and metastasis in vivo in immunodeficient orthotopic mouse models of human breast cancer cell MDA-MB-231.

Compound Libraries for Lin28 1632

Lin28 1632 is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Epigenetics Library and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for Lin28 1632

M. Wt	281.31
Formula	C₁₅H₁₅N₅O
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	108825-65-6
PubChem ID	2737312
InChi Key	WPAQLESUVYGUJZ-UHFFFAOYSA-N
Smiles	CN(C1=CC=CC(C2=NN3C(C)=NN=C3C=C2)=C1)C(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Lin28 1632

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	28.13	100
	ethanol	28.13	100

Preparing Stock Solutions for Lin28 1632

The following data is based on the product molecular weight 281.31.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.55 mL	17.77 mL	35.55 mL
5 mM	0.71 mL	3.55 mL	7.11 mL
10 mM	0.36 mL	1.78 mL	3.55 mL
50 mM	0.07 mL	0.36 mL	0.71 mL

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References for Lin28 1632

References are publications that support the biological activity of the product.

Chen LIN28/ let-7/PD-L1 pathway as a target for cancer immunotherapy. Cancer Immunol.Res. 2019 PMID: 30651289
Chen Targeting LIN28B reprograms tumor glucose metabolism and acidic microenvironment to suppress cancer stemness and metastasis. Oncogene 2019 PMID: 30742065
Roos A small-molecule inhibitor of Lin28. ACS Chem.Biol. 2016 PMID: 27548809