Pladienolide B

Name: Pladienolide B
Brand: Tocris Bioscience
SKU: 6070

Tocris Bioscience, part of Bio-Techne | Catalog # 6070

Citations (25)

COA / SDS

mRNA splicing inhibitor; antitumor

Key Product Details

Description:	mRNA splicing inhibitor; antitumor
Chemical Name:	(4R,7R,8S,9E,11S,12S)-8-(Acetyloxy)-4,7-dihydroxy-12-[(1E,3E,5S)-6-[(2R,3R)-3-[(1R,2S)-2-hydroxy-1-methylbutyl]-2-oxiranyl]-1,5-dimethyl-1,3-hexadien-1-yl]-7,11-dimethyloxacyclododec-9-en-2-one
Purity:	≥95% (HPLC)
Molecular Weight:	536.7

View Full Technical Details for Pladienolide B

Technical Data
Citations

Biological Activity for Pladienolide B

Pladienolide B is a mRNA splicing inhibitor that decreases splicing capacity up to 75% in vitro. Pladienolide B directly targets splicesome-associated 130 (SAP130), inhibits splicing factor 3B subunit (SF3B1) and impairs U2 small nuclear ribonucleoprotein (U2 snRNP) interaction with pre-mRNA. Pladienolide B arrests the cell cycle in G₁ and G₂/M phases and displays antitumor activity against gastric cancer cells (IC₅₀ values are 1.6-4.9 nM). Transient treatment with pladienolide B can reprogram pluripotent hESCs into a distinct type of zigotic genome activation (ZGA)-like cells (ZLCs). Cell permeable and active in vivo.

Technical Data for Pladienolide B

M. Wt	536.7
Formula	C₃₀H₄₈O₈
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	445493-23-2
PubChem ID	90909513
InChi Key	SDOUORKJIJYJNW-UHFFFAOYSA-N
Smiles	C[C@@H](/C=C/[C@H](OC(C)=O)[C@](C)(O)CC[C@@H](O)C1)[C@@H](/C(C)=C/C=C/[C@@H](C)C[C@@H]2[C@@H]([C@H](C)[C@@H](O)CC)O2)OC1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Pladienolide B

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	1

Preparing Stock Solutions for Pladienolide B

The following data is based on the product molecular weight 536.7.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.02 mM	93.16 mL	465.81 mL	931.62 mL
0.1 mM	18.63 mL	93.16 mL	186.32 mL
0.2 mM	9.32 mL	46.58 mL	93.16 mL
1 mM	1.86 mL	9.32 mL	18.63 mL

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References for Pladienolide B

References are publications that support the biological activity of the product.

Li Capturing totipotency in human cells through spliceosomal repression. Cell 2024 PMID: 38843832
Pederiva Splicing controls the ubiquitin response during DNA double-strand break repair. Cell Death Differ. 2016 PMID: 27315300
Sato High antitumor activity of pladienolide B and its derivative in gastric cancer. Cancer Sci. 2014 PMID: 24635824
Lee Therapeutic targeting of splicing in cancer. Nature Medicine