Temozolomide

Name: Temozolomide
Brand: Tocris Bioscience
SKU: 2706
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 2706

(1)

COA / SDS

DNA-methylating antitumor agent

Key Product Details

Description:	DNA-methylating antitumor agent
Alternative Names:	NSC 362856,CCRG 81045
Chemical Name:	3,4-Dihydro-3-methyl-4-oxoimidazo[5,1-d]-1,2,3,5-tetrazine-8-carboxamide
Purity:	≥99% (HPLC)
Molecular Weight:	194.15

View Full Technical Details for Temozolomide

Technical Data
Citations / Reviews (1)

Biological Activity for Temozolomide

Temozolomide is a DNA methylating, chemotherapeutic agent. Displays antitumor activity against a board spectrum of tumors, including leukemias, lymphomas and solid tumors (IC₅₀ = 5.0 μM for cytotoxicity against mouse TLX5 lymphoma cells). Induces autophagy in malignant glioma cells. Exhibits enhanced activity when used in combination with CHC (Cat No. 5029).

Compound Libraries for Temozolomide

Temozolomide is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data for Temozolomide

M. Wt	194.15
Formula	C₆H₆N₆O₂
Storage	Store at RT
Purity	≥99% (HPLC)
CAS Number	85622-93-1
PubChem ID	5394
InChi Key	BPEGJWRSRHCHSN-UHFFFAOYSA-N
Smiles	CN1N=NC2=C(N=CN2C1=O)C(N)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Temozolomide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	9.71	50

Preparing Stock Solutions for Temozolomide

The following data is based on the product molecular weight 194.15.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	10.30 mL	51.51 mL	103.01 mL
2.5 mM	2.06 mL	10.30 mL	20.60 mL
5 mM	1.03 mL	5.15 mL	10.30 mL
25 mM	0.21 mL	1.03 mL	2.06 mL

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Reconstitution Calculator

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References for Temozolomide

References are publications that support the biological activity of the product.

Kanzawa Role of autophagy in temozolomide-induced cytotoxicity for malignant glioma cells. Cell Death Differ. 2004 PMID: 14713959
Clark Antitumor imidazotetraazines. 32. Synthesis of novel imidazotetrazinones and related bicyclic heterocycles to probe the mode of action of the antitumor drug temozol. J.Med.Chem. 1995 PMID: 7739008
Bodell Formation of DNA adducts and induction of lacI mutations in big blue rat-2 cells treated with temozolomide: implications for the treatment of low-grade adult and pediatric tumors. Cancer Epidemiol.Biomarkers Prevent. 2003 PMID: 12815001
Plowman Preclinical antitumor activity of temozol. in mice: efficacy against human brain tumor xenografts and synergism with 1,3-bis(2-chloroethyl)-1-nitrosourea. Cancer Res. 1994 PMID: 8033099