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Simple Plex Assays for Bioprocess Testing

See how Simple Plex assays on the Ella platform can help automate your process impurity testing workflow and get the data you need quickly and reliably. 

Identifying the most promising therapeutic candidates requires a highly sensitive immunoassay solution capable of detecting residual impurities with superior speed, precision and accuracy. Automated Simple Plex assays on Ella offer a consistent, reliable, hands-free solution designed to deliver fast and accurate quantitative results without the hassle of long and complicated workflows.    

Simple Plex Bioprocessing Assays

To meet increasing throughput and safety demands, fast and reproducible detection of host cell proteins (HCPs) is essential during the manufacturing process of a biotherapeutic. The reduction in manual steps inherent in Ella’s automated workflow enables a high level of reproducibility and data quality — all with a method that’s fast and easy to transfer.

Bulk Protein Ribbon

Simple Plex Protein A Assay

Quickly and accurately detect and quantify Protein A impurities with the Simple Plex Protein A assay on Ella. The assay offers an automated solution to get the data you need quickly while ensuring consistent and reliable results for your bioprocess workflow.

  • Wide dynamic range reduces sample dilutions while maintaining sensitivity.
  • Less hands-on time with built-in standard curves.
  • Easier sample prep with simplified pretreatment.
Ella instrument with assay cartridge
Scientist prepares a sample to run automated ELISA on Ella.

Rapid Endonuclease Quantification

The Simple Plex Endonuclease assay is a selective and sensitive method for detecting residual endonuclease impurities in bioprocess samples. Specificity testing has demonstrated that this assay detects both Benzonase® and Denarase®.

3D realistic illustration of HEK cells

Simple Plex HCP Assays with Cygnus Technologies

To bring you the best quality immunoassays, we've partnered with the industry leader in bioprocess impurity testing. Cygnus Technologies provides the highest level of validation and characterization of the antibodies that go into their assays, ensuring that HCP coverage is complete and reproducible.

The Simple Plex CHO HCP 3G-1 assay (SPCKB-OT-003714) and HEK 293 HCP 3G (SPCKB-OT-007066) on Ella are based on the same antibodies currently used in the Cygnus CHO HCP ELISA Kit, 3G (F550-1) and HEK 293 HCP ELISA kit, 3G Resupply 1 (F650S), respectively. These antibodies have been evaluated for reactivity to more than 1,000 individual HCPs present in CHO or HEK 293 strains by state-of-the-art Antibody Affinity Extraction and mass spectrometry methods.

Accelerate Your Process Impurity Testing with Ella

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Precise Quantification

Obtain superior assay specificity and sensitivity at each stage of the purification process. Sub-picogram level detection and 4+ logs of dynamic range provide confirmation that residual contaminants are within required limits.

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Consistent Results

Get consistent, accurate assay results as part of an efficient, hands-free workflow. Advanced microfluidic circuitry performs every step of the assay process, reducing variability and minimizing user error.

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Higher Throughput

Boost your process development throughput with fully validated results in 90 minutes or less. Detect and quantify process impurities with superior speed, accuracy and consistency not possible with a traditional ELISA.

Ella helps GSK Establish Walk-Away Method for CHO HCP Impurity Analysis

Biopharmaceutical analytical scientists at GSK use Ella to accurately quantitate HCP concentrations throughout process development. Read this Biotechniques publication to learn how Ella is allowing their scientists to quickly report CHO HCP trends and speed up process-related decision-making and batch release of drug product.

Benzonase® is a registered trademark of Merck KGaA Corporation. Denarase® is a registered trademark of c-LEcta GmbH