High Sensitivity Multiplexing and Total Protein Normalization

The understanding of cell signaling pathways has significant potential to advance therapies in cancer and regenerative medicine. Simple Western™ from ProteinSimple, a Bio-Techne brand, is a capillary-based automated immunoassay that separates proteins by Charge or Size for specific and sensitive protein characterization using conventional Western blot antibodies.

• Unlike the traditional Western blot, Simple Western assays generate reproducible and fully quantifiable data in as little as 3 hours. And it's all automated.
• RePlex™ on Simple Western performs two sequential immunoassays in the same capillary, allowing users to quantify the relative phospho-isoforms to total protein isoforms of any target without the need to strip & reprobe.
• Take multiplexing even further with chemiluminescence, infrared (IR), and near-infrared (NIR) detection channels.
• Stop the use of housekeeping controls, which can fluctuate in response to cell stimuli with misleading results. Instead, use built-in Total Protein Detection for more accurate quantification of your phospho/pan protein pair in response to cell stimuli.

Analyze an Entire Cell Signaling Pathway Overnight

Cell signaling analysis can be performed in each of your samples in a single experiment. Simple Western can analyze up to 96 samples in a single overnight run using only 5 μL of sample, which is ideal for gaining a comprehensive overview of many targets together with their phosphorylated isoforms in a complex cell signaling pathway in a single experiment. Follow our sample prep workflow for large sample numbers, then load your samples in Peggy Sue, Sally Sue, or NanoPro 1000 and let Simple Western take care of the rest!

Unraveling the Cell Signaling Pathway in Stem Cells

Learn how high sensitivity multiplex assays on Simple Western monitor iPSC differentiation into three primary germ layers of human embryos using GMP proteins and GMP small molecules. Reveal and quantify changes in the expression of pluripotent factors and cell signaling proteins that regulate stem cell differentiation with only 3 μL of starting material. Get unrivaled multiplex flexibility, sensitivity, and reproducibility for accurate protein expression measurements that scale with manufacturing workflows for stem cell therapy and regenerative medicine.

Single-Cell Western Blot Analysis

Single-Cell Westerns on Milo™ perform Western analysis on approximately 1000 individual cells to provide insight into population heterogeneity. Like Simple Western, Single-Cell Western uses conventional Western blot antibodies and is capable of resolving phosphorylated isoforms that are critical for understanding the activity of cell signaling networks. Taken together with Simple Western, these advanced Western blotting solutions offer protein characterization at both a population-level and single-cell resolution.

Uncover Phospho-Protein Heterogeneity with Milo

Single-Cell Westerns on Milo perform measurements that are difficult or impossible to make with conventional westerns or flow cytometry. Learn how Milo can simultaneously quantify phospho- and total protein expression in each individual cell and quantify how the extent of phosphorylation varies across stimulated and unstimulated cell populations.