Rapid Characterization of Immune Response to SARS-CoV-2 Antigens

The SARS-CoV-2 Multi-Antigen Serology Module (SA-001) for use with Jess™ and Abby™ automated Simple Western™ systems enables the detection of human IgG antibodies in human serum or plasma reactive against multiple SARS-CoV-2 antigens.

• Quantify differences in human antibody binding using five SARS-CoV-2 antigens in each sample.
• Minimal set-up time, hands-free operation, and fully analyzed results on 24 samples in 3 hours.
• Access your results remotely to minimize time in the lab for social distancing.

The Simple Western SARS-CoV-2 serological assay is validated for human serum and plasma. Proof-of-concept studies have also shown that the SARS-CoV-2 Antigen Serological Assay can be used to monitor the COVID-19 immune response in animal systems such as mice when the appropriate secondary antibody is chosen.

Figure 1. With this antigen down serological assay, you can quantitatively characterize serum or plasma human IgG antibodies reactive against recombinant Nucleocapsid Protein (N), S1 receptor binding domain protein (RBD), S1 subunit full length, S2 subunit full length, and Spike (S1+S2) viral antigens within one 3-hour run!

The SARS-CoV-2 Multi-Antigen Serology Module for Simple Western provides an immune fingerprint of serum antibodies against five viral antigens of interest for characterization of the COVID-19 immune response in only three hours. As little as 1 µL of serum can be utilized for each patient sample.

Figure 2. The Simple Western SARS-CoV-2 serological assay demonstrates differential human serum reactivity to N, S1 RBD, S1 full length, S2 full length, and Spike (S1+S2) COVID-19 viral antigens by quantification of percent peak area for SARS-CoV-2 antigens detected by PCR positive human serum.