Human beta-Catenin APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC13292A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry, ICC
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 196624
Product Summary for Human beta-Catenin APC-conjugated Antibody
Immunogen
E. coli-derived recombinant human beta-Catenin
Ala2-Leu781
Accession # P35222
Ala2-Leu781
Accession # P35222
Specificity
Detects human, mouse, and rat beta-Catenin in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human beta-Catenin APC-conjugated Antibody
Detection of beta‑Catenin in HeLa Human Cell Line by Flow Cytometry.
HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human beta-Catenin APC-conjugated Monoclonal Antibody (Catalog # IC13292A, filled histogram) or isotype control antibody (Catalog # IC003A, open histo-gram). To facilitate intracellular staining, cells were fixed with 4% para-formaldehyde and permeabilized with Methanol. View our protocol for Staining Intracellular Molecules.Applications for Human beta-Catenin APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with 4% paraformaldehyde and permeabilized with Methanol.
Sample: HeLa human cervical epithelial carcinoma cell line fixed with 4% paraformaldehyde and permeabilized with Methanol.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: beta-Catenin
beta-Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as a transcriptional co-activator in the nucleus and is involved the canonical Wnt signal transduction pathways.
Alternate Names
bCatenin, CTNNB1
Gene Symbol
CTNNB1
UniProt
Additional beta-Catenin Products
Product Documents for Human beta-Catenin APC-conjugated Antibody
Product Specific Notices for Human beta-Catenin APC-conjugated Antibody
For research use only
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