HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human beta-Catenin PE-conjugated Monoclonal Antibody (Catalog # IC13292P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Intracellular Molecules.

Applications for Human beta-Catenin PE-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: beta-Catenin

beta-Catenin is a 94-96 kDa member of the beta-Catenin family, armadillo-repeat superfamily of intracellular molecules. Human beta-Catenin is 781 amino acids (aa) in length and contains three basic regions; there is a "flexible" N-terminus (aa 1-110) that undergoes phosphorylation, O-linked glycosylation, acetylation and ubiquitination, a central region (aa 14-670) that contains 12 armadillo-like repeats and engages in protein-protein interaction, and a "flexible" C-terminus that also undergoes phosphorylation. beta-Catenin is widely, if not ubiquitously expressed, being found in endothelial cells, NKT cells, cortical neurons, neural stem cells, osteoblasts, fibroblasts, skeletal muscle cells, and Tregs. beta-Catenin is perhaps best known as a facilitator for Wnt signaling. In this context, and in the absence of Wnt signaling, non-nuclear or E-Cadherin-associated beta-Catenin is continually synthesized and degraded via a phosphorylation-dependent program. Upon Wnt engagement of its LPR:Fzd receptor complex, phosphorylation stops, allowing for an increase in it half-life and translocation into the nucleus. Here, it binds to TCF, generating a gene-activating transcriptional complex. Notably, Wnt signaling has been described as being beta-Catenin and Ca⁺⁺/non-beta -Catenin based. It now appears they work in tandem, with Ca⁺⁺ mobilization facilitating the translocation of beta-Catenin across the nuclear membrane. Human and mouse full-length beta-Catenin shows 99% aa sequence identity.

Alternate Names

bCatenin, CTNNB1

Entrez Gene IDs

1499 (Human); 12387 (Mouse); 84353 (Rat)

Gene Symbol

CTNNB1

UniProt

P35222

Additional beta-Catenin Products

Product Documents for Human beta-Catenin PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human beta-Catenin PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human beta-Catenin PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC13292P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all beta-Catenin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human beta-Catenin PE-conjugated Antibody

Detection of beta‑Catenin in HeLa Human Cell Line by Flow Cytometry.