OLLAS Epitope Tag Antibody (L2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06713
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Cited:
Nematode - Caenorhabditis elegans
Applications
Validated:
Chromatin Immunoprecipitation Sequencing, Immunoblotting, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Chip Cytometry, ELISA, IF/IHC, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunoprecipitation, Mass Spectrometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 kappa Clone # L2
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
OLLAS (SGFANELGPRLMGK)-tagged extracellular domain of mouse Langerin
Clonality
Monoclonal
Host
Rat
Isotype
IgG1 kappa
Scientific Data Images for OLLAS Epitope Tag Antibody (L2) - BSA Free
Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - C. elegans whole nematode. OLLAS::PRMT-1 Western Blot using NBP1-06713SS (1:1000). Western blot image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Live cell imaging of GFP-tagged MES-2/EZH2 (the catalytic subunit of C. elegans PRC2), and immunofluorescence of H3K27me3 and H3.3/oncohistone (tagged with OLLAS Epitope Tag) in pachytene nuclei of H3-like K27M oncohistone (H3-like mut) worms. Scale bars represent 5 um. Chromosome X was identified by depletion of H3.3 and H3K4me3 staining shown in Supplementary Fig. 3, and is marked with an asterisk. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license Image using the Biotin format of this antibody.
Immunohistochemistry: OLLAS Epitope Tag Antibody (L2) - BSA Free [NBP1-06713] - The A08a neuron receives arbor-specific synaptic inputs. Posterior view of a single A08a labeled by MultiColor FlpOut (MCFO), visualized by A08a-Gal4 > UAS-MCFO. Image using the DyLight 650 form of this antibody (NBP1-06713C).Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31012844/) licensed under a CC-BY license.
Applications for OLLAS Epitope Tag Antibody (L2) - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation Sequencing
reported in scientific literature (PMID 31175278)
Immunoblotting
reported in scientific literature (PMID 27600537)
Immunocytochemistry/Immunofluorescence
1:100
Immunohistochemistry
1:10 - 1:100
Immunohistochemistry-Frozen
1:10 - 1:100
Immunoprecipitation
1:10 - 1:500
Western Blot
1:500 - 1:1000
Reviewed Applications
Read 2 reviews rated 4.5 using NBP1-06713 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: OLLAS Epitope Tag
Alternate Names
E.coli OmpF Linker and mouse Langerin fusion Sequence, OLLA-2
Additional OLLAS Epitope Tag Products
Product Documents for OLLAS Epitope Tag Antibody (L2) - BSA Free
Product Specific Notices for OLLAS Epitope Tag Antibody (L2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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