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Key Product Details

Species Reactivity

Epitope Tag

Applications

Chromatin Immunoprecipitation Sequencing, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

DyLight 650 (Excitation = 652 nm, Emission = 672 nm)

Antibody Source

Monoclonal Rat IgG1 kappa Clone # L2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

OLLAS (SGFANELGPRLMGK)-tagged extracellular domain of mouse Langerin

Clonality

Monoclonal

Host

Rat

Isotype

IgG1 kappa

Scientific Data Images for OLLAS Epitope Tag Antibody (L2) [DyLight 650]

Immunohistochemistry: OLLAS Epitope Tag Antibody (L2) [DyLight 650] [NBP1-06713C] - The A08a neuron receives arbor-specific synaptic inputs. Posterior view of a single A08a labeled by MultiColor FlpOut (MCFO), visualized by A08a-Gal4 > UAS-MCFO. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31012844/) licensed under a CC-BY license.
OLLAS Epitope Tag Antibody (L2) [DyLight 650]

Immunocytochemistry/Immunofluorescence: OLLAS Epitope Tag Antibody (L2) [DyLight 650] [NBP1-06713C] -

The A08a neuron receives arbor-specific synaptic inputs.(A-C'') Light microscopy (point scanning confocal) imaging of A08a neurons. (B) Posterior view of a single A08a labeled by MultiColor FlpOut (MCFO), visualized by A08a-Gal4 > UAS-MCFO. (C-C'') A08a-Gal4 drives expression of UAS-DenMark::mCherry (dendrite marker) and UAS-synaptotagmin::GFP (presynaptic marker). Note the complementary expression in the dendritic and output domains. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31012844), licensed under a CC-BY licence.

Applications for OLLAS Epitope Tag Antibody (L2) [DyLight 650]

Application
Recommended Usage

Chromatin Immunoprecipitation Sequencing

Optimal dilutions of this antibody should be experimentally determined.

Immunoblotting

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry Whole-Mount

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: OLLAS Epitope Tag

The OLLAS (E.coli OmpF Linker and mouse Langerin fusion Sequence) epitope tag is a newly defined tag that is 100-fold more sensitive to other epitope tags tested, including Sigma's anti-FLAG(R) epitope tag antibody. OLLAS is a far superior tag for immunoprecipitation, fluorescent immunohistochemistry and flow cytometry detection.

Alternate Names

E.coli OmpF Linker and mouse Langerin fusion Sequence, OLLA-2

Additional OLLAS Epitope Tag Products

Product Documents for OLLAS Epitope Tag Antibody (L2) [DyLight 650]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for OLLAS Epitope Tag Antibody (L2) [DyLight 650]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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