OLLAS Epitope Tag Antibody (L2) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06713B
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Cited:
Nematode - Caenorhabditis elegans
Applications
Validated:
Immunoblotting, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Chip Cytometry, Western Blot
Label
Biotin
Antibody Source
Monoclonal Rat IgG1 kappa Clone # L2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for OLLAS Epitope Tag Antibody (L2) [Biotin]
Immunogen
OLLAS (SGFANELGPRLMGK)-tagged extracellular domain of mouse Langerin
Reactivity Notes
Tag specific
Clonality
Monoclonal
Host
Rat
Isotype
IgG1 kappa
Scientific Data Images for OLLAS Epitope Tag Antibody (L2) [Biotin]
Western Blot: OLLAS Epitope Tag Antibody (L2) [Biotin] [NBP1-06713B] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Western blot of H3K27me3 and H3.3/oncohistone levels in wild-type, mut, and H3-like mut worms. All versions of H3.3 are tagged with OLLAS Epitope Tag to distinguish them unambiguously form H3. H3 levels are shown as loading control. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license.
Immunocytochemistry/Immunofluorescence: OLLAS Epitope Tag Antibody (L2) [Biotin] [NBP1-06713B] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Live cell imaging of GFP-tagged MES-2/EZH2 (the catalytic subunit of C. elegans PRC2), and immunofluorescence of H3K27me3 and H3.3/oncohistone (tagged with OLLAS Epitope Tag) in pachytene nuclei of H3-like K27M oncohistone (H3-like mut) worms. Scale bars represent 5 um. Chromosome X was identified by depletion of H3.3 and H3K4me3 staining shown in Supplementary Fig. 3, and is marked with an asterisk. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license
Applications for OLLAS Epitope Tag Antibody (L2) [Biotin]
Application
Recommended Usage
Immunohistochemistry-Frozen
1:10-1:100
Western Blot
1:500-1:1000
Application Notes
This OLLAS Epitope Tag antibody is useful for Immunoprecipitation, Western blot and Immunohistochemistry-frozen sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NBP1-06713B in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: OLLAS Epitope Tag
Alternate Names
E.coli OmpF Linker and mouse Langerin fusion Sequence, OLLA-2
Additional OLLAS Epitope Tag Products
Product Documents for OLLAS Epitope Tag Antibody (L2) [Biotin]
Product Specific Notices for OLLAS Epitope Tag Antibody (L2) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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