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Select Recombinant Proteins for Culturing Organoids

 

Proteins for Organoid Culture - Products by Molecule

Activin A*

BMP-2*

BMP-4*

BMP-7

EGF*

FGF-basic*

FGF-7/KGF*

FGF-9

FGF-10

FGF-19

HGF*

Jagged-1

Noggin*

R-Spondin 1

R-Spondin 3

Sonic Hedgehog/Shh*

Wnt-3a*

Wnt-10b

*GMP-grade Proteins are available for these molecules.

Benefits of Using R&D Systems Cytokines and Growth Factors for Organoid Culture

  • High Levels of Biological Activity: Utilizing over 900 different bioassays, the biological activity of every protein we offer is tested in an appropriate bioassay to confirm that it meets our strict QC activity parameters.
  • Lot-to-Lot Consistency: Minimal lot-to-lot variability is ensured by testing each new lot side-by-side with previous lots and with a master lot, so you don’t have to worry whether results will be reproducible over time.
  • High Purity and Low Endotoxin Levels: Our proteins are typically over 95% pure and have a guaranteed industry-leading endotoxin level of <0.1 EU/ug by the LAL method.
  • Highly Cited: R&D Systems proteins commonly used for culturing organoids are highly cited in the scientific literature.
  • Reliable Supply Chain: Our team has the experience and the capacity to ensure that we can provide you with a stable supply of the proteins you need for your research.
  • Bulk Proteins at Discounted Prices: We have the capacity and the expertise to scale up the production of any protein and we offer economical pricing on bulk orders.
  • Custom Protein Capabilities: For specialized protein requests, you can always contact our Custom Protein Services team. We have the expertise and the systems necessary to develop the proteins required to advance your research.
  • Comprehensive Selection of Additional Reagents for Organoid Research: Along with our proteins, Bio-Techne also offers a range of other resources and products for researchers working with organoid systems, including Cultrex™ Basement Membrane Extracts, Tocris™ small molecules, media, media supplements, antibodies, and RNAscope™ ISH assays.
Protein Ribbons in Vials

R&D Systems Recombinant Proteins Are Rigorously Tested for Bioactivity and Lot-to-Lot Consistency to Ensure Optimal and Reproducible Organoid Culture Conditions

Data showing competitor comparison and lot-to-lot consistency testing for R&D Systems Recombinant Human Wnt-3a protein

R&D Systems Recombinant Human Wnt-3a Protein is 1.7-fold More Active than a Leading Competitor’s Recombinant Human Wnt-3a and Displays High Lot-to-Lot Consistency. (A) The bioactivity of R&D Systems Recombinant Human Wnt-3a (Catalog # 5036-WN; orange line) or recombinant human Wnt-3a from another company (green line) was assessed by measuring the ability of the proteins to induce alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line. The ED50 for this effect for R&D Systems Recombinant Human Wnt-3a was 1.7-fold better than the leading competitor’s Wnt-3a protein, with more than twice the maximum response. (B) The lot-to-lot consistency of R&D Systems Recombinant Human Wnt-3a (Catalog # 5036-WN) was assessed by testing the ability of three independent lots of the protein to induce alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line. Each trace on the graph represents data obtained from Recombinant Human Wnt-3a from a different manufacturing run.

Data showing competitor comparison and lot-to-lot consistency testing for R&D Systems Recombinant Human Noggin protein

R&D Systems Recombinant Human Noggin Protein is 30-fold More Active than a Leading Competitor’s Recombinant Human Noggin and Displays High Lot-to-Lot Consistency. (A) The bioactivity of R&D Systems Recombinant Human Noggin (Catalog # 6057-NG; orange line) or recombinant human Noggin from another company (green line) was assessed by measuring the ability of the proteins to inhibit alkaline phosphatase production induced by 50 ng/mL Recombinant Human BMP-4 (R&D Systems, Catalog # 314-BP) in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect for R&D Systems Recombinant Human Noggin was approximately 30-fold greater than the leading competitor’s Noggin protein. (B) The lot-to-lot consistency of R&D Systems Recombinant Human Noggin (Catalog # 6057-NG; orange, light blue, purple lines) was assessed by testing the ability of three independent lots of the protein to inhibit BMP-4-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Each trace on the graph represents data obtained from Recombinant Human Noggin from a different manufacturing run. The dark blue line on the graph is data showing the ability of Recombinant Human BMP-4 (R&D Systems, Catalog # 314-BP; 50 ng/mL) to induce alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line.

Culture and Characterization of iPSC-derived Human Intestinal Organoids

– Part A: iPSC-derived human intestinal organoids stained with antibodies for Vimentin and Desmin and counterstained with DAPI; Part B: iPSC-derived human intestinal organoids stained with antibodies for E-Cadherin and MUC2 and counterstained with DAPI

Culture and Characterization of iPSC-derived Human Intestinal Organoids. iPSC-derived human intestinal organoids were embedded in Cultrex™  UltiMatrix RGF Basement Membrane Extract (R&D Systems, Catalog # BME001-05) and cultured in intestinal organoid culture medium, which includes Recombinant Human Wnt-3a (R&D Systems, Catalog # 5036-WN), Recombinant Human Noggin (R&D Systems, Catalog # 6057-NG), Recombinant Human R-Spondin 1 (R&D Systems, Catalog # 4645-RS), and Recombinant Human EGF (R&D Systems, Catalog # 236-EG), along with the other reagents listed in the intestinal organoid culture medium recipe in the human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (R&D Systems, Catalog # MAB2105; green) and a Goat Anti-Human/Mouse Desmin Antigen Affinity-purified Polyclonal Antibody (R&D Systems, Catalog # AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (Tocris Bioscience, Catalog # 5748; blue). (B) Human intestinal organoids were stained using a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (R&D Systems, Catalog # AF748; green) and a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (Tocris Bioscience, Catalog # 5748; blue).

Culture and Characterization of Adult Stem Cell-derived Human Lung Organoids

Adult Stem Cell-derived Human Lung Organoids

Culture and Characterization of Adult Stem Cell-derived Human Lung Organoids. Adult stem cells isolated from human lung biopsy tissue were embedded in Cultrex UltiMatrix RGF Basement Membrane Extract (R&D Systems, Catalog # BME001-05) and cultured for 20-60 days in lung organoid expansion medium, which includes Recombinant Human R-Spondin 1 (R&D Systems, Catalog # 4645-RS), Recombinant Human Noggin (R&D Systems, Catalog # 6057-NG), Recombinant Human FGF-7 (R&D Systems, Catalog # 251-KG), and Recombinant Human FGF-10 (R&D Systems, Catalog # 345-FG), along with the other reagents listed in the lung organoid expansion medium recipe in the human lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various cell types of the lung. Lung organoids were stained with (A) a Rabbit Anti-Human Cytokeratin 5 (KRT5) Monoclonal Antibody (Novus Biologicals, Catalog NB110-56916; green) and a Goat Anti-Human p63/TP73L Polyclonal Antibody (R&D Systems, Catalog # AF1916; red) to visualize basal cells, (B) a Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (R&D Systems, Catalog # AF1916; red) to visualize basal cells, and (C, D) a Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (R&D Systems, Catalog # MAB2018; red). All samples were counterstained with DAPI (Tocris Bioscience, Catalog # 5748; blue). 

Additional Organoid-Related Products

Organoids and 3D Cell Teaser Solo

Organoid and 3-D Cell Culture Products

Optimize your organoid culture conditions by using our complete range of products designed to support robust, reproducible organoid expansion. Along with R&D Systems proteins, we provide Cultrex basement membrane extracts, Tocris™ small molecules, media, media supplements, and antibodies and RNAscope™ in situ hybridization assays for identifying lineage-specific markers.

Stem Cell Research Teaser

Stem Cell Research Products

Minimize variability in your stem cell experiments and ensure high performance by using our stem cell culture reagents. From isolation and culture to verification, reprogramming, differentiation, and characterization, we have the products that you need for your stem cell research.

Cultrex Basement Membrane Extracts Teaser Solo

Cultrex Basement Membrane Extracts

Browse our wide selection of Cultrex Basement Membrane Extracts and purified extracellular matrix proteins. We offer multiple different formats of Cultrex BME to meet your application-specific needs. Our newest BME, Cultrex UltiMatrix RGF BME, has been optimized to specifically improve the performance and consistency or your spheroid, organoid, and stem cell cultures.

iPSC expansion medium, animal component-free

ExCellerate iPSC Expansion Medium

ExCellerate iPSC Expansion Media supports the robust expansion and maintenance of pluripotent stem cells for enhanced culture consistency and reproducibility. This media is animal component-free, requires no growth factor supplementation, and maintains stable cell integrity over long-term culture.

Tocris GMP small molecules

Small Molecules for Organoid Culture

Safeguard your research with our bioactive small molecules, which are chemically-defined, easy-to-use and have low lot-to-lot variability. Browse our small molecules for use in organoid culture.  

stem cells in culture at Bio-Techne

CEPT Cocktail for Improved Cell Survival

Improve cell survival during organoid generation with our small molecule CEPT cocktail. Comprised of Chroman 1, Emricasan, Lyophilized Polyamine Supplement and trans-ISRIB, the CEPT cocktail enhances the viability of stem cells during passaging, single cell cloning, gene editing, cryopreservation, and differentiation and can be used in the manufacture of organoids for use in disease modeling or drug screening.