Quality is at the Core of Our Immune Response Profiling Immunoassays
Quality at the core means you can depend on our immunoassays for consistent, high-quality immune response profiling data over the long term.
- Vertical Integration: Immunoassays are built with in-house antibodies and diluents, thus guaranteeing a long-term supply of consistent and reliable immunoassays.
- Rigorous Quality Assurance and Quality Control Processes: Our immunoassays uphold the highest standards of consistency, linearity, recovery, and specificity
- 30 Years of Immunoassay Development and Manufacturing Experience: Partner with us for your custom immune response profiling needs.
Explore Immunoassays for Secreted Cytokines
Choose the Assay That Best Suits Your Immune Response Profiling Needs
Simple Plex™ Immunoassays
Simple Plex™ Immunoassays
This hands-free, automated immunoassay has factory-built standards, allowing for easy comparisons across users and geographies. Quantify up to 8 target analytes simultaneously in 90 minutes!
Simple Western Serology Assays
Simple Western Serology Assays
Hands-free serology assays on Simple Western add size separation for quantitative immune response profiling of multiple antigens simultaneously with just 3 µL of serum sample.
Luminex® Assays
Luminex® Assays
Use this flexible, cost-effective multiplex assay to quantify up to 50 analytes per sample in cell culture supernates, serum, plasma, and other fluids. Learn More About Luminex Discovery Assays and High-Performance Assays.
Quantikine™ ELISA Kits
Quantikine™ ELISA Kits
Quantikine ELISA kits are the most cited ELISA on the market. These vertically integrated ELISAs undergo a rigorous Quality Control/Quality Assurance regimen. Choose Quantikine High Sensitivity ELISAs for low-abundance analytes.
DuoSet™ ELISAs
DuoSet™ ELISAs
This flexible ELISA is adaptable to a variety of platforms. It also allows you maximum control over assay conditions. Choose from over 1000 assays across 16 species.
Proteome Profiler™ Antibody Arrays
Proteome Profiler™ Antibody Arrays
Use this simple, qualitative screening assay to simultaneously measure up to 119 cytokines in supernates and cellular lysates.
Bio-Techne's Immunoassay Platforms are Highly Correlated
Bio-Techne maintains consistent quality standards across all our immunoassays. This enables you to move seamlessly from one immunoassay platform to the next as your needs change. In this example, serum IFN-γ detection is highly correlated across Bio-Techne’s immunoassay platforms. A. The Quantikine High Sensitivity (HS) ELISA is highly correlated with Simple Plex Assay (R2=0.99). B. Similarly, HS ELISA data is also highly correlated with Luminex assay data (R2=0.97). C. Finally, data collected using the Simple Plex immunoassay is highly correlated with the Luminex Assay (R2=0.98).
The vertebrate immune system's role is to protect against pathogenic organisms such as viruses or bacteria. It also protects against aberrant cells and plays a role in tissue maintenance and repair1. Investigators use immune response profiling to capture a snapshot of immune system activity in healthy and pathological contexts. Immune response profiling can include assessments of immune cell phenotye, cellular activity, and secreted products such as cytokines and antibodies. This page highlights immunoassays for quantifying secreted cytokines.
Purchase Key Cytokine Immunoassays
Bio-Techne has over 1000 immunoassays for immune response profiling. Are you running a Luminex assay? Use the Luminex Assay Customization Tool to design and purchase your assay. Running an ELISA, ELISpot, or Array? Use the search bar above to find the immunoassay that best suits your needs. We have listed key cytokines below for your convenience.
Luminex Human Discovery Assays - Preconfigured Panels
Luminex Discovery assays are ideal for early-stage discovery or screening. Use the Luminex Assay Customization Tool or adapt the preconfigured panels below to meet your specific needs.
- CD8 T Cell
- Cytokine/Chemokine
- Cytokine/Chemokine 2
- Sepsis
- Sepsis 2
- Soluble Cytokines
- T Cell
- Th1/Th2
- Th 17
High Performance - Fixed Luminex Panels
High Performance Luminex assays are ideal for late-stage target analyte verification. Fixed panels come with pre-selected analytes and are ready to use.
Stocked, Ships in 1-2 Days
High Performance - Configurable Luminex Panels
High Performance Configurable Luminex Panels allow you to customize the panel to meet your specific needs.
Select Analytes, Ships 5-10 Days
These ready-to-use Simple Plex cartridges ship within the continental United States within 48 hours. Choose from single analyte assays, Cytokine Storm panel, Coagulation Panels, or the Cytokine Screening Panel. Need a customized panel for your study? Your local immunoassay specialist is standing by to help!
- IFN-gamma Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
- IL-1b Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays
- IL-2 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
- IL-6 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays| ELISpot Kits
- IL-8 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays
- IL-10 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
- IL-18 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays
- TNF-alpha Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
Profile Cytokines Secreted by Helper T Cells and Regulatory T Cells
CD4+ T cells are subclassified into helper T (Th) cells and regulatory T cells based on their cytokine repertoire2. Although Th1 secreted cytokines are associated with proinflammatory and antitumor responses, Th2 secreted cytokines are correlated with anti-inflammatory responses. Th17 responses play a role in autoimmunity and allergic responses. Finally, Tregs suppress deleterious Th cell activity. It is no surprise that cytokines secreted by Tregs are associated with immunosuppression and cancer progression.
- IFN-gamma Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
- IL-2 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
- IL-12 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kit
- TNF-alpha Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
- IL-4 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELIspot Kits
- IL-5 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
- IL-6 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays| ELISpot Kits
- IL-10 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
- IL-13 Luminex Assays | Simple Plex Assays | ELISA Kits| Proteome Profiler Arrays | ELISpot Kits
Cytokine Release Syndrome Profiling
Cytokine release syndrome (CRS), also known as the cytokine storm, is a life-threatening systemic inflammatory syndrome. It is characterized by the excessive release of cytokines3. CRS is triggered by a variety of events, including cancer, cell therapy, and infections triggered by bacteria or viruses.
Cell Therapy stimulated CRS is characterized by the excessive release of cytokines including4:
- IFN-gamma Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
- IL-6 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays| ELISpot Kits
- IL-8 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays
- IL-10 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
- IL-15 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays
- MCP-1 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays
Severe COVID-19 disease can be characterized by CRS. Cytokines elevated in COVID-stimulated CRS include5:
- G-CSF Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays
- GM-CSF Luminex Assays | Simple Plex Assays | ELISA Kits| Proteome Profiler Arrays
- IFN-gamma Luminex Assays |Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
- IL-1 beta Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays
- IL-6 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays| ELISpot Kits
- IL-7 Luminex Assays | Simple Plex Assays | ELISA Kits
- IL-8 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays
- IL-9 DuoSet ELISAs
- IL-10 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
- IL-17 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
- MCP-1 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays
- TNF-alpha Luminex Assays | Simple Plex Assays |ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
Tocilizumab and Sarilumab have been encouraged for the treatment of critically ill COVID-19 patients. Bio-Techne has Research Grade Human IL-6R alpha (Sarilumab Biosimilar) antibody for research applications.
Systemic inflammatory responses and CRS can be triggered by a variety of stressors including, infection, trauma, ischemia, and more6.
- CRP Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays
- IFN-gamma Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
- IL-1 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays
- IL-1 beta Luminex Assays | Simple Plex Assays | ELISA Kits| Proteome Profiler Arrays
- IL-4 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELIspot Kits
- IL-6 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays| ELISpot Kits
- IL-8 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays
- IL-10 Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
- TNF-alpha Luminex Assays | Simple Plex Assays | ELISA Kits | Proteome Profiler Arrays | ELISpot Kits
Immune Response Profiling Publication Spotlights
Download these publications to see how your peers are using Bio-Techne's immunoassays in scientific and clinical research studies.
Individuals with pre-existing conditions are often excluded from COVID-19 vaccine trials, creating a gap in our understanding of vaccine efficacy. To address this issue, 12 Italian healthcare centers collaborated to understand vaccine efficacy in a large transdisease cohort that was excluded from vaccine trials. Download this publication to see how Simple Plex automated immune response profiling assays were used in the VAX4FRAIL study.
In this peer-reviewed Science Advances paper, investigators used Quantikine™ ELISAs and Proteome Profiler™ Antibody Arrays to understand the relationship between a novel biomarker candidate, acetylated 676th lysine transforming growth factor–beta-induced protein (TGFBIp K676Ac), the cytokine storm, and COVID-19 severity. Download our publication spotlight, highlighting this paper on R&D Systems.com.
In this Nature paper, investigators used Simple Plex immunoassays and the R&D Systems Th9/Th17/Th 22 Discovery Performance Assay to profile cytokines in patients with COVID-19 and dengue haemorrhagic fever (DHF). Their data indicate that these patients have a complex cytokine milieu that varies as a function of disease type and progression. Download the publication to learn more.
SARS-CoV-2 pathophysiology is incompletely understood. To address this issue, De Biasi et al., examined the T cell compartment in severe COVID-19 patients. The authors used the Human Cytokine Discovery Panel to profile 31 cytokines in severe COVID-19 patients. Their data highlighted, among other things, the importance of IL-17 in severe COVID-19 patients.
Individuals with lymphoid malignancies (LM) were not included in SARS-CoV-2 mRNA vaccine clinical trials. Since vaccines have reduced efficacy in LM patients, Marasco et al., wanted to evaluate the immune response to the novel SARs-CoV-2 mRNA vaccines. Download this publication to see how the authors used Ella to profile Th1 associated cytokine release in LM patients that received the Moderna mRNA1273 or Pfizer BNT162b2 mRNA vaccines.
Investigators do not have a complete understanding of the risks and benefits of SARS-CoV-2 vaccination for solid organ transplant patients. To address this issue, D'Offizi et al., profiled the immune response to the SARS-CoV-2 mRNA vaccine in liver transplant recipients. Download this publication to see how the authors used Simple Plex assays on the Ella platform to profile the Th 1-associated response to SARS-CoV2 mRNA vaccines in liver transplant recipients.
The impact of disease-modifying treatments (DMTs) for multiple sclerosis (MS) on immune responses to the SARS-CoV-2 vaccine efficacy is unknown. To address this issue, Tortorella et al., profiled serological and T cell responses in MS patients that received DMTs. Download the publication to see how the authors used Simple Plex immunoassays to profile serum IFN-gamma.
Immune Response Profiling Webinars
Profile Immune Responses to COVID with Simple Plex Automated Immunoassays
Watch this webinar to see how Dr. Jesus Bermejo-Marting uses Simple Plex assays on the Ella platform to profile immune responses to COVID-19.
Deep Dive: Luminex Multiplex Assays at Bio-Techne
Unfamiliar with Luminex Technology? This deep-dive, featuring Bio-Techne's own Richard Fuerstenberg explains the technology underlying Luminex immunoassays. Watch this webinar to see why Bio-Techne's Luminex assays provide higher-quality data than the competition.
Identifying and Removing False Positives in Immunoassays
Watch this webinar to see how Bio-Techne avoids false-positive immunoassay data. In this video, our scientists demonstrate how our proprietary diluents and reagents preclude the false-positive pitfalls that ensnare assays from competitors.
Immune Response Profiling Resources
Quantify IFN-Gamma With Confidence
R&D Systems, a Bio-Techne brand, has an extensive array of Interferon Gamma (IFN-gamma) Immunoassays. Read our IFN-gamma application notes and select the immunoassay that best suits your immune response profiling needs.
Flow Cytometry for Immune Cell Phenotyping
Explore Bio-Techne's solutions for Flow Cytometry. Choose from thousands of antibodies and reagents including CyTOF-ready antibodies and multicolor flow cytometry kits. Explore our interactive cell marker tool. Use the Novus Panel Builder to choose compatible reagents.
Gaining an Edge in Autoimmune Disease Research
“The less we rely on manual steps, the more reproducible the results”
– Dr. Melissa Munroe, M.D., Ph.D.
Research Associate, Oklahoma Medical Research Foundation
Custom Immunoassay Services
Custom ELISA Services
Custom ELISA Services
Leverage our industry-leading expertise to develop and manufacture your custom ELISA.
Custom Luminex Services
Custom Luminex Services
Partner with R&D Systems, a Bio-Techne brand to accelerate your biomarker workflow.
Custom Simple Plex Services
Custom Simple Plex Services
Our scientific experts will work closely with you to create custom Simple Plex™ assays or panels capable of detecting your specific analytes of interest and at your required scale.
1. Rimer, J, et al. (2014) Do all creatures possess an acquired immune system of some sort? Bioassays. 36:273. PMID: 24421210
2. Xu, X, et al, (2016) Expression of Th1- Th2- and Th17-associated cytokines in laryngeal carcinoma. Oncology Letters. 12:1941. PMID: 27588143
3. Fajgenbaum, D.C. and June, C.H. (2020) Cytokine Storm. NEJM. 383:2255. PMID: 33264547
4. Neelapu, S.S. (2019) Managing the toxicities of CAR T-cell therapy. Hematol. Oncol. 37(S1):48. PMID: 31187535
5. Pasrija, R. and Naime, M. (2021) The deregulated immune reaction and cytokines release storm (CRS) in COVID-19 disease. Intl. Immunopharmacology. 90:107225. PMID: 33302033
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