Simple Western Size-Based Immunoassays
Table of Contents
- How Simple Western Immunoassays Work
- Available Molecular Weight Ranges & Detection Reagents
- New to Simple Western? We're Here to Help
How Simple Western Assays Work
Simple Western™ immunoassays take place in a capillary. A plate that contains all the required reagents is first prepared with as little as 3 µL of sample per well and placed in Jess™, Abby™, Peggy Sue™, or Sally Sue™. Approximately 40 nL of sample is loaded into the capillary and the proteins are separated by size as they migrate through a stacking and separation matrix. The separated proteins are then covalently bound to the capillary wall via a proprietary, photoactivated capture chemistry, replacing and eliminating the need to transfer protein to a membrane. Target proteins are identified using a primary antibody and detected using either an HRP-conjugated secondary antibody and chemiluminescent substrate or a fluorescently labeled secondary antibody. The resulting signal is detected and quantitated using Compass for Simple Western software.
ReThink, ReUse, RePlex
RePlex™ on Jess and Abby feature allows you to run two sequential immunoassays within the same capillary to give you rich protein characterization data in a single detection channel.
Stellar Fluorescence Detection Modules
Stellar™ NIR/IR Modules on Jess lead the industry in fluorescence detection sensitivity for Western blotting workflows!
Separation Modules |
Detection Modules |
|---|---|
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Chemiluminescence Fluorescence |
Chemiluminescence
Fluorescence with Stellar High Sensitivity
Fluorescence with Standard Sensitivity |
New to Simple Western? We're Here to Help
Simple Western Kit Builder
Our Simple Western Kit Builder will help you design your kit in 5 easy steps!
Choosing the Right Molecular Weight Range
2-40 kDa. Small molecular weight proteins are vulnerable to poor resolution and poor retention in traditional western blotting workflows. With Simple Western, you can now detect challengingly small molecular weight proteins like hormones and cytokines with our optimized low molecular weight separation module. Consistently detect and quantify low molecular weight proteins without having to tag them.
12-230 kDa. Our most popular range as it covers the the molecular weight range for the majority of proteins.
66-440 kDa. Never want to face a ripped gel, overnight transfer, or sky-high CVs for your high molecular weight proteins again? Done! With the 66–440 kDa Master Kit, you can now enjoy all Simple Western has to offer for a greater range of proteins.
