Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (92%), Canine (90%), Chimpanzee (96%), Feline (90%), Orangutan (96%), Porcine (90%), Primate (96%), Rabbit (90%), Sheep (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1285A

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all 53BP1 Antibodies »

Product Summary for 53BP1 Antibody (1285A) - Azide and BSA Free

Immunogen

Recombinant monoclonal 53BP1 Antibody (1285A) was made to amino acids 350 - 400 of human 53BP1 (NP_005648.1).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Chimpanzee (96%), Gorilla (96%), Orangutan (96%), Gibbon (94%), Bovine (92%), Marmoset (92%), Canine (90%), Feline (90%), Porcine (90%), Rabbit (90%), Sheep (90%)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

>90% by SDS PAGE

Scientific Data Images for 53BP1 Antibody (1285A) - Azide and BSA Free

Western Blot: 53BP1 Antibody (1285A) [MAB18771] - Analysis of HEK293 and ZR-75 cell lysates with 1 ug/ml of h53BP1 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. The molecular weight was observed for h53BP1 at approximately 350 kDa (as indicated), and the theoretical molecular weight of the whole endogenous protein is 214 kDa. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry: 53BP1 Antibody (1285A) [MAB18771] - 53BP1 was detected in immersion fixed HeLa cell using 53BP1 Monoclonal Antibody at 3 ug/ml dilution. Cells were stained using the NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei.

Applications for 53BP1 Antibody (1285A) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 53BP1

Tumor protein p53 binding protein 1 (P53-binding protein 1 or 53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase with a theoretical molecular weight of 214 kDa. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 plays a key role in response to DNA damage, acts as a signaling checkpoint during mitosis, and enhances TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 is known as a key substrate for ataxia telangiectasia mutated (ATM) signaling, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-dependent kinase (DNA-PK). 53BP1 relocalizes to discrete foci overlapping with gamma phosphorylated histone H2AX; demarcating DNA double strand breaks (DSBs) sites following exposure to radiation (1). 53BP1 functions downstream of gamma H2AX-dependent proteins that collectively establish ionizing radiation induced foci at DSBs. 53BP1 is downstream of Mre11/Rad50/NBS1 (MRN complex), MDC1, RNF8, RNF168 and HERC2 which recruit 53BP1 to the DSB site, suggesting a role in DNA repair through genomic stability maintenance (2).

References

1.Henry, E., Souissi-Sahraoui, I., Deynoux, M., Lefevre, A., Barroca, V., Campalans, A., . . . Arcangeli, M. L. (2019). Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations. Haematologica. doi:10.3324/haematol.2019.226936

2.Janoshazi, A. K., Horton, J. K., Zhao, M. L., Prasad, R., Scappini, E. L., Tucker, C. J., & Wilson, S. H. (2020). Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst), 85, 102749. doi:10.1016/j.dnarep.2019.102749

Long Name

p53 Binding Protein 1

Alternate Names

p202, TP53BP1

Gene Symbol

TP53BP1

Additional 53BP1 Products

Product Documents for 53BP1 Antibody (1285A) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 53BP1 Antibody (1285A) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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