53BP1 Antibody (6B3E10) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25028
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Canine (93%), Chimpanzee (99%), Equine (91%), Feline (91%), Orangutan (99%), Porcine (91%), Primate (98%), Rabbit (92%). Backed by our 100% Guarantee.
Applications
Validated:
Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 6B3E10
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
53BP1 Antibody (6B3E10) was made to a partial recombinant human 53BP1 (between residues 500-800) expressed in E. coli
Reactivity Notes
Predicted cross-reactivity based on sequence identity: Chimpanzee (99%), Orangutan (99%), Gibbon (98%), Gorilla (98%), Marmoset (95%), Canine (93%), Rabbit (92%), Feline (91%), Equine (91%), Panda (91%), Porcine (91%)
Localization
Nuclear
Marker
DNA Double Strand Break Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
213 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for 53BP1 Antibody (6B3E10) - BSA Free
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody (6B3E10) - BSA Free [NBP2-25028]
Immunocytochemistry/Immunofluorescence: 53BP1 Antibody (6B3E10) [NBP2-25028] - Staining of human U-2 OS cells using anti-53BP1 antibody. Bottom images: human U-2 OS cells were treated with EPE to induce DNA-damage and showed increase in nuclear spots after the induction of DNA damage with EPE. Image from verified customer review.Immunohistochemistry: 53BP1 Antibody (6B3E10) - BSA Free [NBP2-25028]
Immunohistochemistry: 53BP1 Antibody (6B3E10) [NBP2-25028] - Analysis of paraffin-embedded colon cancer tissues using 53BP1 Antibody with DAB staining.Western Blot: 53BP1 Antibody (6B3E10)BSA Free [NBP2-25028]
Western Blot: 53BP1 Antibody (6B3E10) [NBP2-25028] - Western blot analysis using against HEK293 (1) and 53BP1 (AA: 574-773)-hIgGFc transfected HEK293 (2) cell lysate. The observed molecular weight is ~52 kDa and the theoretical molecular weight of the whole endogenous protein is 214 kDa.Applications for 53BP1 Antibody (6B3E10) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:200 - 1:400
Immunocytochemistry/Immunofluorescence
reported by customer review
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Reviewed Applications
Read 1 review rated 5 using NBP2-25028 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: 53BP1
References
1.Henry, E., Souissi-Sahraoui, I., Deynoux, M., Lefevre, A., Barroca, V., Campalans, A., . . . Arcangeli, M. L. (2019). Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations. Haematologica. doi:10.3324/haematol.2019.226936
2.Janoshazi, A. K., Horton, J. K., Zhao, M. L., Prasad, R., Scappini, E. L., Tucker, C. J., & Wilson, S. H. (2020). Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst), 85, 102749. doi:10.1016/j.dnarep.2019.102749
Long Name
p53 Binding Protein 1
Alternate Names
p202, TP53BP1
Entrez Gene IDs
7158 (Human)
Gene Symbol
TP53BP1
UniProt
Additional 53BP1 Products
Product Documents for 53BP1 Antibody (6B3E10) - BSA Free
Product Specific Notices for 53BP1 Antibody (6B3E10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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