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Aminopeptidase N/CD13 Antibody (6E9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26507

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26507-100ul
NBP3-26507-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6E9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Aminopeptidase N/CD13 Antibody (6E9)

Immunogen

A synthesized peptide derived from Human Aminopeptidase N/CD13 [UniProt P15144]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aminopeptidase N/CD13 Antibody (6E9)

Aminopeptidase N/CD13 Antibody (6E9)

Immunohistochemistry: Aminopeptidase N/CD13 Antibody (6E9) [NBP3-26507] -

Immunohistochemistry: Aminopeptidase N/CD13 Antibody (6E9) [NBP3-26507] - Image of Aminopeptidase N/CD13 Antibody (6E9) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Aminopeptidase N/CD13 Antibody (6E9)

Immunohistochemistry: Aminopeptidase N/CD13 Antibody (6E9) [NBP3-26507] -

Immunohistochemistry: Aminopeptidase N/CD13 Antibody (6E9) [NBP3-26507] - Image of Aminopeptidase N/CD13 Antibody (6E9) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for Aminopeptidase N/CD13 Antibody (6E9)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Aminopeptidase N/CD13

Alanine aminopeptidase (CD13) is expressed on the majority of peripheral blood monocytes and granulocytes. CD13 is also expressed by the majority of acute myeloid leukemias, chronic myeloid leukemias in myeloid blast crisis, a smaller percentage of lymphoid leukemias and myeloid cell lines. CD13 is also found in several types of non hematopoietic cells such as fibroblasts and endothelial cells and in a soluble form in blood plasma. CD13 is not expressed on B cells, T cells, platelets or erythrocytes. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. CD13 plays a role in biologically active peptide metabolism, in the control of growth and differentiation, in phagocytosis and in bactericidal/tumoricidal activities. CD13 also serves as a receptor for human coronaviruses.

CD13 is commonly used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. Therefore, CD13 antibodies are useful tools for cancer and renal disorder research.

Alternate Names

Aminopeptidase M, ANPEP, APN, CD13, gp150, PEPN

Gene Symbol

ANPEP

Additional Aminopeptidase N/CD13 Products

Product Documents for Aminopeptidase N/CD13 Antibody (6E9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aminopeptidase N/CD13 Antibody (6E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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