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APC Antibody (KT44)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23227

Novus Biologicals, part of Bio-Techne
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NBP3-23227

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # KT44

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for APC Antibody (KT44)

Immunogen

Clone KT44 was generated from a recombinant protein containing amino acid residues from the central region mouse APC. This sequence is highly conserved in human and rat APC, and has low homology to APC2. [UniProt# Q61315]

Specificity

This antibody detects full length APC at 300 kDa and proteolytic fragments of APC at lower molecular weights. The antibody detects APC or its fragments in SW480, CaCO2, DLD-1, and Hct116 cells. In immunocytochemistry, the antibody detects in APC in clusters at the plasma membrane and at the ends of microtubules.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Theoretical MW

300 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Applications for APC Antibody (KT44)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:250
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: APC

Adenomatous polyposis coli (APC) is a protein that plays a critical role in several cellular processes. APC is a tumor suppressor, regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division and associates with other proteins involved in cell attachment and signaling (1). APC regulates

Long Name

Adenomatosis Polyposis Coli

Alternate Names

DP2, DP3, FPC, GS, Min

Gene Symbol

APC

Additional APC Products

Product Documents for APC Antibody (KT44)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for APC Antibody (KT44)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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