Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Porcine

Applications

Validated:

Immunocytochemistry/Immunofluorescence, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all ATF6 Antibodies »

Product Specifications

Immunogen

A genomic peptide made to an internal region of the human ATF6 protein (within residues 200-350). [Swiss-Prot P18850]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31235574).

Localization

Endoplasmic reticulum membrane, nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATF6 Antibody - BSA Free

Western Blot: ATF6 AntibodyBSA Free [NBP1-75478]

Western Blot: ATF6 AntibodyBSA Free [NBP1-75478]

Western Blot: ATF6 Antibody [NBP1-75478] - Total protein from HeLa cells treated with and without 1 mM DTT for 24 hours was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 1% BSA. The membrane was probed with 2.0 ug/ml anti-ATF6 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the decrease in full length ATF6 (90 kDa, arrow) and increase in cleaved ATF6 (55 kDa, arrowhead) upon DTT treatment and the activation of the unfolded protein response pathway.
Simple Western: ATF6 AntibodyBSA Free [NBP1-75478]

Simple Western: ATF6 AntibodyBSA Free [NBP1-75478]

Simple Western: ATF6 Antibody [NBP1-75478] - Simple Western lane view shows a specific band for ATF6 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.
Immunocytochemistry/ Immunofluorescence: ATF6 Antibody - BSA Free [NBP1-75478]

Immunocytochemistry/ Immunofluorescence: ATF6 Antibody - BSA Free [NBP1-75478]

Immunocytochemistry/Immunofluorescence: ATF6 Antibody [NBP1-75478] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-ATF6 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Applications for ATF6 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/Immunofluorescence

1:100

Simple Western

1:25

Western Blot

1:100-1:2000
Application Notes
This ATF6 antibody is useful for Immunocytochemistry/Immunofluorescence.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 1 using NBP1-75478 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATF6

Endoplasmic reticulum stress results in protein folding irregularities and proteolytic abberations. ER stress is mediated in part by the activating transcription factor (ATF6). ATF6 is a signal transducer that prohibits phosphorylation of certain proteins, like the insulin receptor, and protects overactive responses to ER stress. This ultimately mediates apoptosis through mitochondrial cellular signaling. Influenza infections are known to affect ER stress in lung endothelial cells, partially mediated by ATF6 activity. This protein is also being investigated for potential optimization of recovery efforts, following traumatic injury, especially in spinal cord and brain.

Long Name

Activating Transcription Factor 6

Alternate Names

ACHM7, ATF6 alpha

Entrez Gene IDs

22926 (Human)

Gene Symbol

ATF6

UniProt

P18850 (Human)

Additional ATF6 Products

Product Documents for ATF6 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATF6 Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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