beta-Actin Antibody (AC-15) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-67828
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Drosophila (Negative), Feline, Fish, Guinea Pig, Hamster, Leech, Mammal, Primate, Rabbit, Sheep, Squirrel, Turkey, Zebrafish
Cited:
Human, Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Knockdown Validated, Knockout Validated, Microarray, Simple Western, Western Blot
Cited:
WB
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # AC-15
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This beta-Actin Antibody (AC-15) was made to a slightly modified Beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Epitope
N-terminus of the beta isoform of actin.
Reactivity Notes
The antibody reacts with b-actin-expressing cells in bovine, canine, chicken, feline, carp, leech, guinea pig, human, mouse, porcine, rabbit, rat, and sheep. Does not react with amoeba or drosophila. Does not cross react with adult cardiac or skeletal muscle or amoeba beta actin. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
Beta-actin (AC-15). In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
This is the purified version of NB600-501.
Scientific Data Images for beta-Actin Antibody (AC-15) - BSA Free
Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Whole cell extract of human fibroblasts was separated on SDS-PAGE and blotted with Monoclonal Anti-beta-Actin. The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate and AEC substrate. Lanes A: Antibody dilution 1:5,000 B: Negative control (only secondary antibody).
Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Staining in HS-68 cells.
Immunohistochemistry: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Staining in breast, Epithelium 40X.
Applications for beta-Actin Antibody (AC-15) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10-40 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
0.5-1 ug/ml
Application Notes
See Simple Western Antibody Database for Simple Western validation: tested in HeLa lysate; antibody dilution of 1:50; separated by size; detects a band at 54 kDa
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: beta-Actin
References
1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245
2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619
3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968
4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433
Alternate Names
ACTB, betaActin
Gene Symbol
ACTB
Additional beta-Actin Products
Product Documents for beta-Actin Antibody (AC-15) - BSA Free
Product Specific Notices for beta-Actin Antibody (AC-15) - BSA Free
This is the purified version of NB600-501.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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