beta Tubulin Antibody (TU-06)
Novus Biologicals, part of Bio-Techne | Catalog # NB120-7792
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Chicken, Fish, Plant
Cited:
Rat, Plant
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Single Cell Western, Western Blot
Cited:
WB
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Clone # TU-06
Concentration
1.0 mg/ml
Product Summary for beta Tubulin Antibody (TU-06)
Immunogen
Beta-subunits of porcine brain tubulin.
Reactivity Notes
Expected species cross-reactivity based on sequence homology: Carp, Bovine, Hamster, S.Cerevisiae, S.Pombe, and Protozoa. Plant reactivity reported in scientific literature (PMID: 26594217).
Localization
Cytoplasmic
Specificity
The antibody TU-06 recognizes an epitope (aa 81-95) on phylogenetically conserved N-terminal structural domain of beta-tubulin (recognizes all beta-tubulin isoforms) in various species.
Marker
Microtubule Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Scientific Data Images for beta Tubulin Antibody (TU-06)
Western Blot: beta Tubulin Antibody (TU-06) [NB120-7792] - Analysis (reducing conditions) of HPB-ALL human peripheral blood leukemia cell line. Lane 1: negative control. Lane 2,3,4,5,6: immunostaining with anti-beta-tubulin (TU-06; dilution 0,5 ug/ml, 1 ug/ml, 2 ug/ml, 4 ug/ml, 5 ug/ml).
Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (TU-06) [NB120-7792] - Staining of 3T3 mouse embryonal fibroblast cell line using anti-beta-tubulin (TU-06) (detection by Goat anti-mouse IgM Cy(R)5). Nucleus is stained with DAPI (blue).
Applications for beta Tubulin Antibody (TU-06)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunocytochemistry: fixed and permeabilized cells; positive control: 3T3 mouse embryonal fibroblast cell line. Immunohistochemistry (paraffin sections) positive tissue: heart.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NB120-7792 in the following applications:
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
TBS (pH 8.0)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: beta Tubulin
Long Name
Tubulin beta chain
Alternate Names
OK/SW-cl.56, TUBB, TUBB5
Gene Symbol
TUBB
UniProt
Additional beta Tubulin Products
Product Documents for beta Tubulin Antibody (TU-06)
Product Specific Notices for beta Tubulin Antibody (TU-06)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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