Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Cell depletion, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # PK-136

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD161/NK1.1 Antibodies »

Product Summary for CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

Immunogen

The original antibody was generated by immunizing (C3H X BALB)F1 mice with spleen cells enriched for NK-1 cells and bone marrow cells from CE mice.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

ELISA: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] -

ELISA: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] - Binding curves of the rabbit IgG chimeric version of the Anti-CD161/NK1.1 antibody PK-136 (NBP3-20165; blue line) and isotype control (NBP2-52638, anti-fluorescein; black line) to an ELISA plate coated with CD161/NK1.1 mIgG1 Fc fusion at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using CD161/NK1.1 antibody. For signal detection, a 1:4000 dilution of a HRP-conjugated anti-rabbit IgG antibody was used.

Applications for CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Cell depletion

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD161/NK1.1

NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation.

Alternate Names

Klrb1c, Ly-59, Ly55c

Gene Symbol

Klrb1c

Additional CD161/NK1.1 Products

Product Documents for CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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