CD161/NK1.1 Antibody (PK136) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-77528
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation
Cited:
FLOW, IF/IHC, IHC, IHC-Fr, IHC-P
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # PK136
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for CD161/NK1.1 Antibody (PK136) - BSA Free
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for CD161/NK1.1 Antibody (PK136) - BSA Free
Flow Cytometry: CD161/NK1.1 Antibody (PK136) [NB100-77528] - Analysis using the PE/Cy7 conjugate of NB100-77528. Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) FITC and staining buffer (autofluorescence) (left) or Anti-Mouse NK1.1 PE-Cy7 (right). Total viable cells were used for analysis.
Flow Cytometry: CD161/NK1.1 Antibody (PK136) [NB100-77528] - Mouse splenocytes were stained with CD49b (DX5) APC and purified NK1.1 (clone PK136) (top) or mouse IgG2a isotype control (bottom), followed by anti-mouse IgG2a FITC.
Flow Cytometry: CD161/NK1.1 Antibody (PK136) [NB100-77528] - C57BL/6 mouse splenocytes stained with purified PK136, followed by anti-mouse IgG2a FITC
Applications for CD161/NK1.1 Antibody (PK136) - BSA Free
Application
Recommended Usage
Flow Cytometry
< 1.0 ug/10^6 cells
Immunocytochemistry/ Immunofluorescence
1:20-1:1000
Immunohistochemistry
1:20-1:1000
Immunohistochemistry-Frozen
1:20-1:1000
Immunohistochemistry-Paraffin
1:20-1:1000. Use reported in scientific literature (PMID 21118981)
Immunoprecipitation
1:20-1:1000
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 0.25 ug/1 x10^6 cells in 100 ul. It is recommended that the reagent be titrated for optimal performance for each application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD161/NK1.1
Additional CD161/NK1.1 Products
Product Documents for CD161/NK1.1 Antibody (PK136) - BSA Free
Product Specific Notices for CD161/NK1.1 Antibody (PK136) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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