Key Product Details

Species Reactivity

Validated:

Human, Mouse, Equine (Negative)

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, In vivo assay, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-28

Format

BSA Free

Concentration

1.0 mg/ml

View all CD45 Antibodies »

Product Specifications

Immunogen

This CD45 Antibody (MEM-28) was prepared from Human thymocytes and T lymphocytes.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27272386). Please note that CD45 Antibody (MEM-28) is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Type I membrane protein

Specificity

CD45 Antibody (MEM-28) reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. HLDA III; WS Code NL 833a

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

147 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD45 Antibody (MEM-28) - BSA Free

Flow Cytometry: CD45 Antibody (MEM-28) - BSA Free [NB500-319]

Flow Cytometry: CD45 Antibody (MEM-28) - BSA Free [NB500-319]

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood lymphocytes with anti-human CD45 antibody (MEM-28) Alexa Fluor 700.
Immunocytochemistry/ Immunofluorescence: CD45 Antibody (MEM-28) - BSA Free [NB500-319]

Immunocytochemistry/ Immunofluorescence: CD45 Antibody (MEM-28) - BSA Free [NB500-319]

Immunocytochemistry/Immunofluorescence: CD45 Antibody (MEM-28) [NB500-319] - staining of human peripheral blood mononuclear cell using anti-human CD45 antibody (MEM-28) (green). DNA visualized by DAPI (blue).
Flow Cytometry: CD45 Antibody (MEM-28) - BSA Free [NB500-319]

Flow Cytometry: CD45 Antibody (MEM-28) - BSA Free [NB500-319]

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 antibody (MEM-28) PE-CyTM5.
View 8 more images

Applications for CD45 Antibody (MEM-28) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug/ml

Immunocytochemistry/Immunofluorescence

10 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:50

Western Blot

1 ug/ml
Application Notes
Western blot: Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS, Non-reducing conditions. SDS-PAGE (6% separating gel). Immunohistochemistry-Paraffin: No pre-treatment of tissue sections is essential. Immunocytochemistry: paraformaldehyde fixation can be used. Use in In-vivo reported in scientific literature (PMID 27272386). This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD45

CD45, also known as leukocyte common antigen (LCA), T200, or Ly5, is a member C of the class 1 receptor-like protein tyrosine phosphatase (PTPRC) family (1, 2). It is a transmembrane glycoprotein which, due to alternative splicing, has a multiple isoforms with a theoretical molecular weight ranging from 180 - 220 kDa (1, 3-5). Human CD45 is synthesized as a 1281 amino acid sequence consisting of an alternatively spliced extracellular receptor-like region, a cysteine-rich domain, fibronectin-like III repeats, a transmembrane segment, and a cytoplasmic region with tandem protein tyrosine phosphatase (PTP) domains: the membrane proximal domain (D1) and the membrane distal domain (D2) (3, 5). CD45 is expressed on all nucleated hematopoietic cells and their precursors, except mature red blood cells, and is one of the most abundantly-expressed cell-surface glycoproteins, comprising approximately 10% of surface proteins in lymphocytes (3). Functionally, CD45 is essential for development and activation of T-cells and B-cells (1-5). More specifically, CD45 positively regulates antigen receptor signaling and Src-family member kinase activity (1, 3). There are many ways to regulate CD45 phosphatase activity including ligand binding, dimerization, protein interactions, cellular localization, and covalent modifications (3, 6). Ligands for CD45 include pUL11, a transmembrane protein of the cytomegalovirus RL11 (CMV RL11) family, and placental protein 14 (PP14), both of which exclusively bind CD45, and various lectins including CD22, galectin-1, galectin-3, macrophage mannose receptor (MR), and macrophage galactose-type lectin (MGL) (6).

Given its role in immune cell development and activation, CD45 has also been linked to a variety of diseases. The importance of CD45 in immunity has been revealed in human and mouse studies where CD45-deficiency leads to a severe-combined immunodeficiency (SCID) phenotype (2, 3, 6). A CD45-knockout mice study revealed inhibited thymocyte production and poor B-cell response, whereas CD45 activation in mice causes lymphoproliferation and autoantibody production (3). CD45 variants have been associated with altered immune function and autoimmune disorders including multiple sclerosis, systemic lupus erythematosus (SLE), and rheumatoid arthritis (6). Furthermore, altered CD45 expression has been implicated in oncological conditions including chronic lymphatic leukemia, acute lymphatic leukemia, Hodgkin lymphoma, multiple myeloma, and diffuse large B-cell lymphoma (6). Considering its role in autoimmune disorders, immunodeficiency and cancer, CD45 is an ideal therapeutic target (3, 6). The main approaches to control CD45 function is through either selective inhibitors or anti-CD45 antibodies (3).

Alternative names for CD45 includes B220, CD antigen: CD45, CD45 antigen, CD45R, EC 3.1.3.48, GP180, LCA, Leukocyte common antigen, LY5, protein tyrosine phosphatase receptor type c polypeptide, PTPRC, receptor-type tyrosine-protein phosphatase C, T200 Glycoprotein, and T200.

References

1. Trowbridge, I. S., & Thomas, M. L. (1994). CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. Annual review of immunology. https://doi.org/10.1146/annurev.iy.12.040194.000505

2. Andersen, J. N., Jansen, P. G., Echwald, S. M., Mortensen, O. H., Fukada, T., Del Vecchio, R., Tonks, N. K., & Moller, N. P. (2004). A genomic perspective on protein tyrosine phosphatases: gene structure, pseudogenes, and genetic disease linkage. FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

3. Hermiston, M. L., Xu, Z., & Weiss, A. (2003). CD45: a critical regulator of signaling thresholds in immune cells. Annual review of immunology. https://doi.org/10.1146/annurev.immunol.21.120601.140946

4. Tonks, N. K., Diltz, C. D., & Fischer, E. H. (1990). CD45, an integral membrane protein tyrosine phosphatase. Characterization of enzyme activity. The Journal of biological chemistry.

5. Nam, H. J., Poy, F., Saito, H., & Frederick, C. A. (2005). Structural basis for the function and regulation of the receptor protein tyrosine phosphatase CD45. The Journal of experimental medicine. https://doi.org/10.1084/jem.20041890

6. Rheinlander, A., Schraven, B., & Bommhardt, U. (2018). CD45 in human physiology and clinical medicine. Immunology letters. https://doi.org/10.1016/j.imlet.2018.01.009

Long Name

Cluster of Differentiation 45

Alternate Names

CD45, LCA, PTPRC, T200 Glycoprotein

Gene Symbol

PTPRC

Additional CD45 Products

Product Documents for CD45 Antibody (MEM-28) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD45 Antibody (MEM-28) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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