CD90/Thy1 Antibody (G7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28031

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/Immunofluorescence, In vitro assay

Cited:

IF/IHC, In vitro assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2c Kappa Clone # G7

Format

Azide and BSA Free

Concentration

0.5 mg/ml

View all CD90/Thy1 Antibodies »

Product Summary for CD90/Thy1 Antibody (G7) - Azide and BSA Free

Immunogen

Mouse T cell hybridoma C6/G8

Specificity

Mouse CD90 (Thy-1.1 & Thy-1.2 alloantigens/Thy-1 epitope region A); Mr 28-32 kDa

Marker

Mesenchymal Cell Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2c Kappa

Scientific Data Images for CD90/Thy1 Antibody (G7) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: CD90/Thy1 Antibody (G7) [NBP1-28031] - Primary cortical neurons isolated from postnatal day 7 CD1 mice. Primary antibody incubation overnight at 1:500. Alexa Fluor secondary antibodies at 1:500. Image from a verified customer review.
Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Flow Cytometry: CD90/Thy1 Antibody (G7) [Biotin] [NBP1-28033] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD90-BIOT and Rat Anti-Mouse CD3 epsilon-PE followed by Streptavidin-FITC. Image using the Biotin form of this antibody.
Flow Cytometry: CD90/Thy1 Antibody (G7) [NBP1-28031] - Analysis using the Allophycocyanin conjugate of NBP1-28031. Multiple staining of BALB/c splenocytes.
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Applications for CD90/Thy1 Antibody (G7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000
Application Notes
CD90/Thy1 antibody validated for ICC/IF from a verified customer review. In Vitro Assay reported in (PMID: 2893823).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-28031 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.1M BBS (pH 8.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD90/Thy1

CD90/Thy-1, a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily of cell surface receptors, consists of a single V-set domain. (1-4) It is expressed on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes and neurons of all mouse strains. (1,2) MAb G7 stimulates T-cell proliferation and IL-2 secretion, via signalling through the T-cell receptor/CD3 complex. (1,5-7) The MAb has also been reported to promote apoptosis of thymocytes and CTL clones,4,7 and to mediate adhesion of thymocytes to thymic stroma.8

Alternate Names

CD90, Thy1

Entrez Gene IDs

21838 (Mouse)

Gene Symbol

THY1

UniProt

P01831 (Mouse)

Additional CD90/Thy1 Products

Product Documents for CD90/Thy1 Antibody (G7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD90/Thy1 Antibody (G7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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