CD90/Thy1 Antibody (OX-7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65543

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry Free-Floating, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OX-7

Format

BSA Free

Concentration

1.0 mg/ml

View all CD90/Thy1 Antibodies »

Product Summary for CD90/Thy1 Antibody (OX-7) - BSA Free

Immunogen

Rat Thy 1 antigen

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:28928774).

Marker

Mesenchymal Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD90/Thy1 Antibody (OX-7) - BSA Free

Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus.
Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus with MOUSE ANTI RAT CD90 (THY 1.1).
Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymocytes.
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Applications for CD90/Thy1 Antibody (OX-7) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/Immunofluorescence

1:10-1:500

Immunohistochemistry Free-Floating

reported in scientific literature (PMID 35383427)

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD90/Thy1

CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1. Thy1.1, expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues. Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab' of OX-7 for rat Thy1 is 3 x 10^9m^1 and for mouse Thy1.1 is 3 x 10^8m-^1(1). Clone OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy (5).This clone has been reported to induce glomerular nephritis in Wistar rats (8).This product is routinely tested in flow cytometry on rat thymocytes.

Alternate Names

CD90, Thy1

Entrez Gene IDs

21838 (Mouse); 24832 (Rat); 100339279 (Rabbit)

Gene Symbol

THY1

UniProt

P01830 (Rat)

Additional CD90/Thy1 Products

Product Documents for CD90/Thy1 Antibody (OX-7) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD90/Thy1 Antibody (OX-7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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