COX5b Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89499

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-89499-25ul
NBP1-89499
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all COX5b Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ASGGGVPTDEEQATGLEREIMLAAKKGLDPYNVLAPKGASGTREDPNLVPSISNKRIVGCICEEDNTSVVWFWLHKGEAQRCPRCGAHYKLVP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for COX5b Antibody

Western Blot: COX5b Antibody [NBP1-89499]

Western Blot: COX5b Antibody [NBP1-89499]

Western Blot: COX5b Antibody [NBP1-89499] - Western blot analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: COX5b Antibody [NBP1-89499]

Immunocytochemistry/ Immunofluorescence: COX5b Antibody [NBP1-89499]

Immunocytochemistry/Immunofluorescence: COX5b Antibody [NBP1-89499] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: COX5b Antibody [NBP1-89499]

Immunohistochemistry-Paraffin: COX5b Antibody [NBP1-89499]

Immunohistochemistry-Paraffin: COX5b Antibody [NBP1-89499] - Staining of human stomach shows distinct granular cytoplasmic positivity in glandular cells.
View 1 more images

Applications for COX5b Antibody

Application
Recommended Usage

Immunocytochemistry/Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: COX5b

Alternate Names

COXVB, Cytochrome c oxidase polypeptide Vb, cytochrome c oxidase polypeptide VB, mitochondrial, cytochrome c oxidase subunit 5B, mitochondrial, cytochrome c oxidase subunit Vb

Gene Symbol

COX5B

Additional COX5b Products

Product Documents for COX5b Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for COX5b Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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