Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all DCLK1 Antibodies »

Product Summary for DCLK1 Antibody - BSA Free

Immunogen

Antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human DCLK1. The immunogen is located within the last 50 amino acids of DCLK1. Amino Acid Squence: IRRGRFSDEDATRM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DCLK1 Antibody - BSA Free

Western Blot: DCLK1 Antibody [NBP1-77127] - Analysis of DCLK1 expression in rat brain tissue lysate with DCLK1 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/Immunofluorescence: DCLK1 Antibody [NBP1-77127] - Staining of DCLK1 in mouse brain tissue with DCLK1 Antibodyat 20 ug/mL.
Immunohistochemistry: DCLK1 Antibody [NBP1-77127] - Staining of DCLK1 in mouse brain tissue with DCLK1 Antibodyat 5 ug/mL.
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Applications for DCLK1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5-1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DCLK1

DCLK1 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK1 mRNA, like that of the homologous DCLK2 and DCLK3, is highly expressed in adult brain, but only DCLK1 and DCLK2 transcripts are present in human fetal brain and the developing mouse embryo, suggesting that DCLK1 and DCLK2 may play roles in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a gene that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization. Unlike DCLK1, DCLK2 expression does not change in DCX-null mice.

Long Name

Doublecortin-like and CAM Kinase-like 1

Alternate Names

CL1, CLICK1, DCAMKL1, DCDC3A

Gene Symbol

DCLK1

UniProt

NP_004725 (Human)

Additional DCLK1 Products

Product Documents for DCLK1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DCLK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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