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Key Product Details

Species Reactivity

Epitope Tag

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

ELISA, ICC/IF, WB

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Goats were immunized with E-Tag (GAPVPYPDPLEPR) conjugated to KLH.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for E-Tag Epitope Tag Antibody - BSA Free

Western Blot: E-Tag Epitope Tag Antibody [NB600-518] - Detection of E-Tag-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-E-Tag antibody NB600-518 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for E-Tag Epitope Tag Antibody - BSA Free

Application
Recommended Usage

ELISA

Coating-1:100-1:500 Primary-1:1000-1:30000

Immunocytochemistry/ Immunofluorescence

1:100 - 1:400

Immunoprecipitation

1 - 4 ug/mg lysate

Western Blot

1:1000 - 1:30000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: E-Tag Epitope Tag

The E-Tag is a popular epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized E-tag epitope represents the peptide sequence GAPVPYPDPLEPR.

Alternate Names

E epitope tag, GAPVPYPDPLEPR epitope tag, GAPVPYPDPLEPR tag

Additional E-Tag Epitope Tag Products

Product Documents for E-Tag Epitope Tag Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for E-Tag Epitope Tag Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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