Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 5E10

Format

BSA Free

Concentration

1 mg/ml

View all eIF2 alpha Antibodies »

Product Specifications

Immunogen

Recombinant human eIF2 alpha/EIF2S1 (1-315aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for eIF2 alpha/EIF2S1 Antibody (5E10) - BSA Free

Western Blot: eIF2 alpha/EIF2S1 Antibody (5E10)BSA Free [NBP1-97936]

Western Blot: eIF2 alpha/EIF2S1 Antibody (5E10)BSA Free [NBP1-97936]

Western Blot: eIF2 alpha/EIF2S1 Antibody (5E10) [NBP1-97936] - The extracts of HeLa (50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (5E10) - BSA Free [NBP1-97936]

Immunocytochemistry/ Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (5E10) - BSA Free [NBP1-97936]

Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (5E10) [NBP1-97936] - Analysis of EIF2S1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human EIF2S1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western Blot: eIF2 alpha/EIF2S1 Antibody (5E10)BSA Free [NBP1-97936]

Western Blot: eIF2 alpha/EIF2S1 Antibody (5E10)BSA Free [NBP1-97936]

Western Blot: eIF2 alpha/EIF2S1 Antibody (5E10) [NBP1-97936] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: Jurkat cell lysate Lane 3.: NIH-3T3 cell lysate

Applications for eIF2 alpha/EIF2S1 Antibody (5E10) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF2 alpha

EIF2S1, also known as eIF2a, is the alpha subunit of the translation initiation factor eIF2 complex which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. The phosphorylation state of eIF2S1 controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2S1 is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation. Recombinant human EIF2S1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Eukaryotic Translation Initiation Factor 2 Alpha

Alternate Names

EIF2S1

Gene Symbol

EIF2S1

UniProt

NP_004085 (Human)

Additional eIF2 alpha Products

Product Documents for eIF2 alpha/EIF2S1 Antibody (5E10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eIF2 alpha/EIF2S1 Antibody (5E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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