eIF2 alpha/EIF2S1 Antibody (OTI3H4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02669
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Monkey, Primate
Applications
Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI3H4
Concentration
0.69 mg/ml
Product Summary for eIF2 alpha/EIF2S1 Antibody (OTI3H4)
Immunogen
Full length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
35.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for eIF2 alpha/EIF2S1 Antibody (OTI3H4)
Western Blot: EIF2S1 Antibody (3H4) [NBP2-02669] Analysis of extracts (35ug) from 9 different cell lines by using anti-eIF2A monoclonal antibody.
Immunocytochemistry/Immunofluorescence: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY eIF2A.
Immunohistochemistry-Paraffin: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of paraffin-embedded Human prostate tissue using anti-eIF2A mouse monoclonal antibody.
Applications for eIF2 alpha/EIF2S1 Antibody (OTI3H4)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/Immunofluorescence
1:100
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:1000-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.69 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: eIF2 alpha
Long Name
Eukaryotic Translation Initiation Factor 2 Alpha
Alternate Names
EIF2S1
Entrez Gene IDs
1965 (Human)
Gene Symbol
EIF2S1
Additional eIF2 alpha Products
Product Documents for eIF2 alpha/EIF2S1 Antibody (OTI3H4)
Product Specific Notices for eIF2 alpha/EIF2S1 Antibody (OTI3H4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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