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Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [Alexa Fluor® 350]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52644AF350

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Label

Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 2.4G2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse BALB/c Macrophage J774 Cell Line.

Reactivity Notes

Mouse

Specificity

This antibody binds to an epitope on the extracellular domains of the mouse Fc(gamma)III and Fc(gamma)II, thereby inhibiting their ability to bind antibodies via their Fc region. receptors. Also binding the Fc(gamma)I receptor (CD64) via its Fc domain.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [Alexa Fluor® 350]

CD16/CD32 Antibody (2.4G2) [Alexa Fluor® 350]

CD16/CD32 Antibody (2.4G2) [Alexa Fluor® 350] [NBP2-52644AF350] -

CD16/CD32 Antibody (2.4G2) [Alexa Fluor® 350] [NBP2-52644AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.

Applications for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [Alexa Fluor® 350]

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Fc gamma RII/RIII (CD32/CD16)

Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. In humans each group of receptors is encoded by 2 - 3 closely related genes designated A, B, and C. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Different Fc receptors can function as activators, as inhibitors, or as decoy receptors.

Long Name

Fc gamma Receptor II/III

Alternate Names

CD32/CD16

Gene Symbol

FCGR3A

Additional Fc gamma RII/RIII (CD32/CD16) Products

Product Documents for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [Alexa Fluor® 350]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) [Alexa Fluor® 350]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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