Key Product Details

Species Reactivity

Mouse

Applications

Block/Neutralize, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 93

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all CD16/CD32 Antibodies »

Product Specifications

Immunogen

Murine CD16/32 (CD16/Fc gamma III and CD32/FcgammaII receptors)

Specificity

Murine CD16/32 (CD16/Fc gamma III and CD32/Fc gamma II receptors)

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for CD16/CD32 Antibody (93) - Azide and BSA Free

Flow Cytometry: CD16/CD32 Antibody (93) [NBP1-27946] - Analysis using the PerCP/Cy5.5 conjugate of NBP1-27946. Staining of BALB/c splenocytes with 0.06 ug of Rat IgG2a K Isotype Control PerCP-Cy5.5 (open histogram) or 0.06 ug of Anti-Mouse CD16/CD32 PerCP-Cy5.5 (filled histogram). Total viable cells were used for analysis.
Flow Cytometry: CD16/CD32 Antibody (93) [NBP1-27946] - Staining of mouse splenocytes with Anti-Mouse CD16/CD32 APC (left), and PE-Cy7 (right). Autofluorescence is shown via open histogram. Total cells were used for analysis.
Flow Cytometry: CD16/CD32 Antibody (93) [NBP1-27946] - Analysis using the PE conjugate of NBP1-27946. Multiple staining of BALB/c splenocytes.
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Applications for CD16/CD32 Antibody (93) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

<= 3 ug/10^6 cells

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD16/CD32

The lymphocyte Fcgamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. (1, 2) The Fcgamma type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. (1) The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression.4 Besides identifying FcgammaR+ cells, monoclonal antibodies to the FcgammaII/III receptor have been used to block Fc receptor binding of IgG, Fc mediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on FcgammaR for parasitic invasion. (4-6) The monoclonal antibody 93 recognizes a conformational epitope formed by FcgammaII and FcgammaIII receptors.

Alternate Names

CD16, CD16A, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), FCG3, FCGR3, FCGR3A, FCGRIII, FCR-10, FCRIII, FCRIIIA, IGFR3

Gene Symbol

FCGR3A

Additional CD16/CD32 Products

Product Documents for CD16/CD32 Antibody (93) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD16/CD32 Antibody (93) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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