Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # FAP/4851

Concentration

0.2 mg/ml

View all Fibroblast Activation Protein alpha/FAP Antibodies »

Product Summary for Fibroblast Activation Protein alpha/FAP Antibody (FAP/4851)

Immunogen

Recombinant fragment (around aa 1-200) of human Fibroblast Activation Protein alpha/FAP protein (exact sequence is proprietary) (Uniprot: Q12884)

Localization

Cell surface, Cell membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14219)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Scientific Data Images for Fibroblast Activation Protein alpha/FAP Antibody (FAP/4851)

Immunohistochemistry-Paraffin: Fibroblast Activation Protein alpha/FAP Antibody (FAP/4851) [NBP3-13883] - Formalin-fixed, paraffin-embedded human placenta stained with Fibroblast Activation Protein alpha/FAP antibody (FAP/4851). Inset: PBS instead of primary antibody, secondary only negative control.
Protein Array: Fibroblast Activation Protein alpha/FAP Antibody (FAP/4851) [NBP3-13883] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Fibroblast Activation Protein alpha/FAP Antibody (FAP/4851).

Applications for Fibroblast Activation Protein alpha/FAP Antibody (FAP/4851)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Fibroblast Activation Protein alpha/FAP

FAP (fibroblast activation protein-alpha; also known as seprase) is a type II transmembrane serine protease that degrades gelatin, Type I collagen, and N-terminal prolines. FAP is induced on reactive stromal fibroblasts in epithelial cancers, sarcomas, and granulation tissue, and may play roles in tumor invasion, tissue remodeling, and wound repair.

Alternate Names

FAP, Seprase

Gene Symbol

FAP

UniProt

Q12884 (Human)

Additional Fibroblast Activation Protein alpha/FAP Products

Product Documents for Fibroblast Activation Protein alpha/FAP Antibody (FAP/4851)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Fibroblast Activation Protein alpha/FAP Antibody (FAP/4851)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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