Fibroblast Activation Protein alpha/FAP Antibody

Western Blot: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - Western blot using affinity purified anti-Fibroblast Activation protein alpha/Fibroblast Activation Protein alpha/FAP antibody shows detection of Fibroblast Activation Protein alpha/FAP protein in whole cell lysates from Fibroblast Activation Protein alpha/FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected Fibroblast Activation Protein alpha/FAP. Primary antibody was used at 1:1,000.

ELISA: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - ELISA results of purified Fibroblast Activation protein alpha/FAP antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.