Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-68095B

Novus Biologicals, part of Bio-Techne
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NB110-68095B
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # SE-1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Hepatic Sinusoidal Endothelial Cells Antibodies »

Product Specifications

Immunogen

Rat Hepatic Sinusoidal Endothelial Cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Applications for Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [Biotin]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) is useful for Immunohistochemistry and Western blot, where a band can be seen around 45-50 kDa. For IHC use neutral buffered formalin fixated (perfusion fixation is recommended) paraffin embedded tissues after proteinase K treatment or acetone fixed frozen sections.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Hepatic Sinusoidal Endothelial Cells

Among the vascular endothelial cells, hepatic sinusoidal endothelial (HSE) cells are morphologically unique because they have open fenestrations in their cytoplasm and lack basal lamina. This unique structure is assumed to facilitate the exchange and transport of various substances between blood and hepatocytes through the space of Disse. It has been suggested that the formation of this structure is regulated by hepatocyte-related soluble factors or by the extracellular matrix. This antibody has observed no cross-reactivity with other types of endothelial cells or non-endothelial cells. By immunoelectron microscopy, the antigen recognized by this antibody was localized to the membrane surface of HSE. This antibody may be a useful marker to study the role of HSE in various pathophysiological conditions of the liver.

Alternate Names

Hepatic cells, Hepatic Sinusoidal Endothelial Cells, HSE, SE1, Sinusoidal Endothelial Cells

Additional Hepatic Sinusoidal Endothelial Cells Products

Product Documents for Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Hepatic Sinusoidal Endothelial Cells Antibody (SE-1) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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