Human ABCB5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5799
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Summary for Human ABCB5 Antibody
Immunogen
E. coli-derived recombinant human ABCB5
Ile141-Val247
Accession # Q2M3G0
Ile141-Val247
Accession # Q2M3G0
Specificity
Detects human ABCB5 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human ABCB5 Antibody
Detection of Human ABCB5 by Western Blot.
Western blot shows lysates of Bowes human melanoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human ABCB5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5799) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ABCB5 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human ABCB5 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Bowes human melanoma cell line and K562 human chronic myelogenous leukemia cell line
Sample: Bowes human melanoma cell line and K562 human chronic myelogenous leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ABCB5
ABCB5 (ATP-binding cassette, subfamily B [DR/TAP] member 5) is a 90 kDa (predicted MW) member of the human P-glycoprotein family of molecules. It is expressed by CD133+ pluripotent pigment stem cells, where it serves as a marker for cells that show a high incidence of polyploidy. Human ABCB5 is 812 amino acids (aa) in length. It is possibly a five transmembrane protein with a 247 aa N-terminal extracellular domain (ECD) (aa 1-247) and a 283 aa C-terminal cytoplasmic region (aa 530-812). The ECD contains one ABC transporter (aa 2-177), while the cytoplasmic region contains a second ABC transporter (aa 570-808). There are two potential splice forms. One shows a seven aa substitution for aa 125-812, while another possesses an alternative start site 445 aa upstream of the standard site. Over aa 141-247, human ABCB5 shares 64% aa identity with mouse ABCB5.
Long Name
ATP-binding Cassette, Sub-family B (MDR/TAP), Member 5
Alternate Names
ABCB5 P-gp, ABCB5alpha, ABCB5beta, ATP-binding cassette protein, ATP-binding cassette sub-family B member 5, ATP-binding cassette, sub-family B (MDR/TAP), member 5, EC 3.6.3, EC 3.6.3.44, EST422562, P-glycoprotein ABCB5
Gene Symbol
ABCB5
UniProt
Additional ABCB5 Products
Product Documents for Human ABCB5 Antibody
Product Specific Notices for Human ABCB5 Antibody
For research use only
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