Human APC C-Terminus Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3695
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Summary for Human APC C-Terminus Antibody
Immunogen
E. coli-derived recombinant human APC
Ser2537-Val2843
Accession # P25054
Ser2537-Val2843
Accession # P25054
Specificity
Detects human APC C-Terminus in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human APC C-Terminus Antibody
APC in Human Colon Cancer Tissue.
APC was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using 15 µg/mL Goat Anti-Human APC C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3695) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human APC C-Terminus Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue
Western Blot
0.1 µg/mL
Sample: Recombinant Human APC C-Terminus
Sample: Recombinant Human APC C-Terminus
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: APC
Long Name
Adenomatosis Polyposis Coli
Alternate Names
DP2, DP3, FPC, GS, Min
Gene Symbol
APC
UniProt
Additional APC Products
Product Documents for Human APC C-Terminus Antibody
Product Specific Notices for Human APC C-Terminus Antibody
For research use only
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