Human CD3 epsilon Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB100F
Clone UCHT1 was used by HLDA to establish CD designation
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry, Functional Assay
Label
Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # UCHT1
Product Summary for Human CD3 epsilon Fluorescein-conjugated Antibody
Immunogen
Human thymocytes (1)
Specificity
Recognizes the epsilon-chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD3 epsilon Fluorescein-conjugated Antibody
Detection of CD3 epsilon in Human Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either Mouse Anti-Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F) or (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.Applications for Human CD3 epsilon Fluorescein-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood lymphocytes
Sample: Human peripheral blood lymphocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD3 epsilon
CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
References
- Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
Alternate Names
CD3e
Gene Symbol
CD3E
Additional CD3 epsilon Products
Product Documents for Human CD3 epsilon Fluorescein-conjugated Antibody
Product Specific Notices for Human CD3 epsilon Fluorescein-conjugated Antibody
For research use only
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