Human EGFR Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF231

R&D Systems, part of Bio-Techne
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BAF231
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AF231
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA Detection (Matched Antibody Pair), Flow Cytometry, Western Blot

Cited:

ELISA Detection, ELISA Microarray Development, Functional Assay, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all EGFR Antibodies »

Product Summary for Human EGFR Biotinylated Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human EGFR
Leu25-Ser645
Accession # CAA25240

Specificity

Detects human EGFR in ELISAs and Western blots. In ELISAs, this antibody shows less than 3% cross-reactivity with recombinant mouse EGFR and less than 0.1% cross-reactivity with recombinant human (rh) EGF, rhErbB2, and rhErbB3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human EGFR Biotinylated Antibody

Detection of EGFR antibody in A431 Human Cell Line antibody by Flow Cytometry.

Detection of EGFR in A431 Human Cell Line by Flow Cytometry.

A431 human epithelial carcinoma cell line was stained with Human EGFR Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF231, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).

Applications for Human EGFR Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: A431 human epithelial carcinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human EGFR (Catalog # 1095-ER)

Human EGFR Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human EGFR Antibody (Catalog # AF231)
  • Standard: Recombinant Human EGFR Protein (Catalog # 1095-ER)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.3 using BAF231 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

The epidermal growth factor receptor (EGFR) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER2), ErbB3 (HER3), and ErbB4 (HER4). All family members are type I transmembrane glycoproteins that have an extracellular domain which contains two cysteine-rich domains separated by a spacer region that is involved in ligand binding, and a cytoplasmic domain which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain. EGFR has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, heparin-binding EGF and neuregulin-2 alpha in the absence of a co-receptor. Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation and cell signaling. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGFR signaling has also been shown to play a role in carcinogenesis (1-3).

References

  1. Daly, R.J. (1999) Growth Factors, 16:255.
  2. Schlessinger, J. (2000) Cell. 103:211.
  3. Maihle, N.J. et al. (2002) Cancer Treat. Res. 107:247.
 

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

UniProt

CAA25240

Additional EGFR Products

Product Documents for Human EGFR Biotinylated Antibody

Product Datasheets

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Product Specific Notices for Human EGFR Biotinylated Antibody

For research use only

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